Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 4315752..4316293 Replicon chromosome
Accession NZ_CP080273
Organism Mycolicibacterium smegmatis strain Nishi

Toxin (Protein)


Gene name mazF Uniprot ID A0A2U9PVI3
Locus tag KZ781_RS20505 Protein ID WP_003895797.1
Coordinates 4315964..4316293 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0R0N3
Locus tag KZ781_RS20500 Protein ID WP_011729843.1
Coordinates 4315752..4315961 (+) Length 70 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ781_RS20475 (KZ781_20325) 4311023..4311793 - 771 WP_011729837.1 SDR family oxidoreductase -
KZ781_RS20480 4312330..4312731 - 402 Protein_4067 cupin domain-containing protein -
KZ781_RS20485 (KZ781_20335) 4312761..4313681 - 921 WP_233046304.1 NADP-dependent oxidoreductase -
KZ781_RS20490 (KZ781_20340) 4313678..4313962 - 285 WP_011729841.1 hypothetical protein -
KZ781_RS20495 (KZ781_20345) 4314158..4315591 + 1434 WP_233046718.1 dihydrolipoyl dehydrogenase -
KZ781_RS20500 (KZ781_20350) 4315752..4315961 + 210 WP_011729843.1 antitoxin MazE family protein Antitoxin
KZ781_RS20505 (KZ781_20355) 4315964..4316293 + 330 WP_003895797.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KZ781_RS20510 (KZ781_20360) 4316336..4316899 - 564 WP_014878038.1 helix-turn-helix domain-containing protein -
KZ781_RS20515 (KZ781_20365) 4317005..4317940 + 936 WP_003895799.1 alpha/beta fold hydrolase -
KZ781_RS20520 (KZ781_20370) 4317937..4319649 + 1713 WP_011729844.1 NAD(P)/FAD-dependent oxidoreductase -
KZ781_RS20530 (KZ781_20380) 4319866..4320246 + 381 WP_029104585.1 phage holin family protein -
KZ781_RS20535 (KZ781_20385) 4320264..4321289 + 1026 WP_014878040.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-104)

Antitoxin

Antitoxin MazE-like

(5-68)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 11739.52 Da        Isoelectric Point: 10.4023

>T211561 WP_003895797.1 NZ_CP080273:4315964-4316293 [Mycolicibacterium smegmatis]
VRRGDIYTAAARGAYTGKPRPVLIIQDDRFDATASVTVVPFTTSDTDAPLLRIRIEPTPATGLSTVSSLMIDKVTTVPRS
SLTHRVGRLAETDMVRTDRALLVFLGIAG

Download         Length: 330 bp

>T211561 NZ_CP104965:c1193248-1192973 [Devosia neptuniae]
ATGCCATATGAATGGGACGAGGCCAAGAACCGCGCCAACCGGCTCAAGCACGGTATCAGCTTTGAGGAAGCGCAACTGAT
TTTCGCCGGTCCGGTCTTTTCCTGGGTCGATACCCGTCTCGACTATGGCGAGACCAGAATTGTGAGCATCGGCCTGATAA
GCGGAATCGTGGCGGTCACGGTGGTGCATACCGACCGTAATGGCACCACTCGCATCATCTCAGCGCGACTGGCCAACCAG
CGGGAAAGGACGATTTATGACGACCATCTCCGATAA

Antitoxin


Download         Length: 70 a.a.        Molecular weight: 8114.98 Da        Isoelectric Point: 6.2231

>AT211561 WP_011729843.1 NZ_CP080273:4315752-4315961 [Mycolicibacterium smegmatis]
MTPARDRVRRHRERLRRQGLRPVQIWVPDVNAPEFRREAHRQSELVAAGEHEAEDQAFVDAISVDWDDA

Download         Length: 210 bp

>AT211561 NZ_CP104965:c1192992-1192741 [Devosia neptuniae]
ATGACGACCATCTCCGATAAACGTCTGGCCGAACTGGCCAAGCGCCGCGACGAGCAGATCGACTATAGCGACATCCCCGA
ACTGGACGAACAATTTTGGGCCAATGCCCAATTGGTCGAGCCGGACGGCACGCAGCAGGTGACATTGCGCGTCAAGAAGT
CAGTTCTCGAGGCCTATAAGGCCACCGGCAAGGGCTACCAGACCCGCATGAACGCGGTGCTGGAATCCTATGCCAAGACC
CTGCGGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U9PVI3

Antitoxin

Source ID Structure
AlphaFold DB A0R0N3

References