Detailed information of TA system TA211560

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 3271040..3272036
• Replicon: chromosome
• Accession: NZ_CP080273
• Organism: Mycolicibacterium smegmatis strain Nishi

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: A0A2U9PRH2
• Locus tag: KZ781_RS15585
• Protein ID: WP_003894880.1
• Coordinates: 3271458..3272036 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: I7GB97
• Locus tag: KZ781_RS15580
• Protein ID: WP_003894879.1
• Coordinates: 3271040..3271432 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ781_RS15560 (KZ781_15430)	3266511..3267629	+	1119	WP_003894875.1	acyltransferase	-
KZ781_RS15565 (KZ781_15435)	3267802..3268407	+	606	WP_003894876.1	class I SAM-dependent methyltransferase	-
KZ781_RS15570 (KZ781_15440)	3268409..3270037	-	1629	WP_239708700.1	GMC family oxidoreductase N-terminal domain-containing protein	-
KZ781_RS15575 (KZ781_15445)	3270347..3270976	+	630	WP_003894878.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
KZ781_RS15580 (KZ781_15450)	3271040..3271432	+	393	WP_003894879.1	ParD-like family protein	Antitoxin
KZ781_RS15585 (KZ781_15455)	3271458..3272036	+	579	WP_003894880.1	zeta toxin family protein	Toxin
KZ781_RS15590 (KZ781_15460)	3272048..3273400	-	1353	WP_003894881.1	alpha/beta hydrolase-fold protein	-
KZ781_RS15595 (KZ781_15465)	3273735..3273890	+	156	WP_003894882.1	hypothetical protein	-
KZ781_RS15600 (KZ781_15470)	3274088..3275476	+	1389	WP_233045776.1	L-serine ammonia-lyase	-
KZ781_RS15605 (KZ781_15475)	3275510..3276070	-	561	WP_058126281.1	hypothetical protein	-
KZ781_RS15610 (KZ781_15480)	3276230..3277036	+	807	WP_239708701.1	transporter substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-136)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21119.30 Da
• Isoelectric Point: 9.5596

>T211560 WP_003894880.1 NZ_CP080273:3271458-3272036 [Mycolicibacterium smegmatis]
VKRLDLVVGPNGAGKSTFVELTLAPLLTGSAFVNADEIAKRRWPHAPAEHSYDAARIASDTRLKLIELGESFIAETVFSH
PSKIELIDTAQAAGYTVVMHVVMIPEDLAVLRVQHRVRAGGHHVPEEKIRERHQRLWPLVALAAARADISTFYDNSAVRG
PRIVAQLAGGFAVGRPAWPMWAPQVLTSRWPE

Nucleotide

• Length: 579 bp

>T211560 NZ_CP104964:c471044-470763 [Devosia neptuniae]
TTGGCAAAGTCATTCAAGGGAATCCAGCTGCAGATCGTAGCGTCCGGCAAGGCGCCGAAGGGCTTCCCTACTGACGTTCT
GCAGGCGGCTCGCCGAAAGCTTTTCATGATCACCAATGCTGCCGCTCTTCAGGACTTGCTGATGCCGCCCGGCAATAAAC
TGGAGGCCTTGAAAGGAAATCGTTCCGGCCAACATTCCATCCGCATCAACGACCAGTGGCGCGTTTGCTTCCGTTGGTCA
AATGGCGCAGCTGAAGACATCGAAATCACCGACTACCACTAG

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 13841.29 Da
• Isoelectric Point: 4.9246

>AT211560 WP_003894879.1 NZ_CP080273:3271040-3271432 [Mycolicibacterium smegmatis]
MTETADRVTRFAADLVESAAAEGARQSRSAKQQLDHWARVGRAVSSQHTAARRRVEAALAGDLALRDLTPEEGVVFNAEI
SAAIEENLAHTDYGQVLAGRGVTTVALDDDGAIVRYQPDGTTTVLAPAQR

Nucleotide

• Length: 393 bp

>AT211560 NZ_CP104964:c470723-470412 [Devosia neptuniae]
ATGTCCTTGACCTTTGACCCGCCAATCCATCCTGGCGAGATTCTACGTGAAGAGTTTCTTGTGCCGCTTAATCTATCGGC
CGGTGCTCTTGCCAAGCGTGTCAGCGTTCCCCGTACGCGGATTGAGCGCATCGTGAAGGAGGAAACGCCGATCACGCCCG
ACACGGCTCTACGTCTGTCGCGCGTGCTAGGGACGACCGCTCAATTCTGGATGAACATGCAGTCCGCATACGATCTGGTG
CGAGCCGAACAGGCCCATTCGGAAGATCTCAATTTGATCGCACCACTAGACAGCCTCGCGAGGGCCAGCTGA

Structures

Toxin

• AlphaFold DB: A0A2U9PRH2

Antitoxin

• AlphaFold DB: A0A2U9PRP8