Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Fic(toxin)
Location 1155757..1156340 Replicon chromosome
Accession NZ_CP080273
Organism Mycolicibacterium smegmatis strain Nishi

Toxin (Protein)


Gene name doc Uniprot ID A0A2U9PKR4
Locus tag KZ781_RS05260 Protein ID WP_003892660.1
Coordinates 1155951..1156340 (+) Length 130 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A2U9PKT9
Locus tag KZ781_RS05255 Protein ID WP_011727559.1
Coordinates 1155757..1155954 (+) Length 66 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ781_RS05235 (KZ781_05180) 1151229..1152658 + 1430 Protein_1041 IS3 family transposase -
KZ781_RS05240 (KZ781_05185) 1152609..1152926 - 318 WP_239708043.1 metallophosphoesterase -
KZ781_RS05245 (KZ781_05190) 1153175..1154545 - 1371 WP_233044278.1 restriction endonuclease -
KZ781_RS05250 (KZ781_05195) 1154702..1155727 - 1026 WP_239708846.1 HNH endonuclease domain-containing protein -
KZ781_RS05255 (KZ781_05200) 1155757..1155954 + 198 WP_011727559.1 type II toxin-antitoxin system antitoxin Phd Antitoxin
KZ781_RS05260 (KZ781_05205) 1155951..1156340 + 390 WP_003892660.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KZ781_RS05265 (KZ781_05210) 1156417..1157847 + 1431 WP_003892661.1 DUF3375 domain-containing protein -
KZ781_RS05270 (KZ781_05215) 1157844..1158422 + 579 WP_038557632.1 DUF4194 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(18-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13868.49 Da        Isoelectric Point: 4.2481

>T211558 WP_003892660.1 NZ_CP080273:1155951-1156340 [Mycolicibacterium smegmatis]
VTEYLDREDVLTAGSIAFGGELKVRDYGLLDAAVARPQATVYGVDAYPRLWDKAAALLQSLARNHALVDGNKRTAWAAAW
TFLHINGVQLAADFDVDRAEDLMNEVATRDCDLDSIAAELAGFAAAQTG

Download         Length: 390 bp

>T211558 NZ_CP104964:168065-168589 [Devosia neptuniae]
ATGCCTGAGTTGGTCGACACAAAGCCGTTCATTCTATGGCGGGCCTACGTTCCCCAGTGGTCATATCTGCCGCTGTCGGG
TGAGGGGGCCGCCCGCTTCGGCGGCCGCTGGAATCCAGTCGGTATCCCGACCATCTACGCGGCGCAGGAATTGTCGACCG
CTTGGGGGGAGTACAATCAGGGGTTCGTTCAACACCCTGCATTGATCGCTCAACTCGAGCTCAAGGGCGCCCGTCTCGTC
GATACGAGGGACCCCGAAATTCTCCAAGAATGGGGACTCGAGGAAGGCATCCACCAATCGGAGTGGCGGTCGGATCTTGA
CAATGGCGCTGTTCCGAGCACGCACGATGCCCACCGGCGATTTGTTGAGGGCGGCCTTGATGGTGTCATCTATCCCTCTT
TCATGTCTCCAGGCGGAAGTTGCGTTGCTCTCTGGCGTTGGAATGATGCGGGAGGTCCCATTCTCAACATTATTGATCCC
GACGGTCGACTTCCGACGTCGCCAGCATCATGGCGAAAGCCCTGA

Antitoxin


Download         Length: 66 a.a.        Molecular weight: 7276.30 Da        Isoelectric Point: 8.9353

>AT211558 WP_011727559.1 NZ_CP080273:1155757-1155954 [Mycolicibacterium smegmatis]
MPSLNIDFDEAEMEQIRAAARADDLSLKKFAHAAVMERASAHKRRVAEAARLVAERSAELNRRLA

Download         Length: 198 bp

>AT211558 NZ_CP104964:167701-168072 [Devosia neptuniae]
ATGCAGTCCCAGGGTCTCGAAATTGGCGCCACCCGTTTTGGCGAGAGTGGTTCGCCTTTCCTGTCGGCGCATCGACTGTC
AGAACAGCTCGGTGTAACGCAATCCGAACTCGCTAAGCTCATCGGTATTGCCCGCAACACCCTCACGGCGAAGTCCGCGA
CCCGAAAGGTGGACGCGGCCCTGAGCCCGATCGTACGCATTCTGGCTATGGCCGCAGAAATGGCCGGCGGCGAAAACCGG
GCGGTGATTTGGTTCAAGCACCAGCCGATCCCCGGTTGGGCGGGCAAGACCGCCTACGATCTTGTCGGGCAAGGCAAGTC
TGATCAGGTTCTTGCTTATCTGGAGGCTGTTCGCTCAGGCGTTTATGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U9PKR4

Antitoxin

Source ID Structure
AlphaFold DB A0A2U9PKT9

References