Detailed information of TA system TA211557

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 5781076..5781717
• Replicon: chromosome
• Accession: NZ_CP080272
• Organism: Mycolicibacterium smegmatis strain Rabinowitchi

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: KZ782_RS27385
• Protein ID: WP_011730672.1
• Coordinates: 5781076..5781519 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: A0A2U9PXD6
• Locus tag: KZ782_RS27390
• Protein ID: WP_003897042.1
• Coordinates: 5781529..5781717 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ782_RS27365 (KZ782_27240)	5776560..5777297	+	738	WP_011730668.1	GntR family transcriptional regulator	-
KZ782_RS27370 (KZ782_27245)	5777299..5778828	+	1530	WP_233046689.1	cyclohexanecarboxylate-CoA ligase	-
KZ782_RS27375 (KZ782_27250)	5778829..5779602	+	774	WP_233046690.1	SDR family oxidoreductase	-
KZ782_RS27380 (KZ782_27255)	5779599..5781071	+	1473	WP_058127741.1	long-chain fatty acid--CoA ligase	-
KZ782_RS27385 (KZ782_27260)	5781076..5781519	-	444	WP_011730672.1	SRPBCC family protein	Toxin
KZ782_RS27390 (KZ782_27265)	5781529..5781717	-	189	WP_003897042.1	antitoxin	Antitoxin
KZ782_RS27395 (KZ782_27270)	5781743..5784118	-	2376	WP_058126983.1	cation-translocating P-type ATPase	-
KZ782_RS27400 (KZ782_27275)	5784132..5785739	-	1608	WP_233046691.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-141)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16157.75 Da
• Isoelectric Point: 9.9432

>T211557 WP_011730672.1 NZ_CP080272:c5781519-5781076 [Mycolicibacterium smegmatis]
MAKLSVSVEVPLPPEQAWEYASDLSRYHEWLSIHRAWRSKLPETLEKGTVIESIVEVKGMLNRVRWTLVHYKPPQALTLN
GDGRGGVKVKLIGKIKPTDNGATVGFDLHLGGPALFGPIGMVVAAALKSDIQESLNRFKQLYAPSAT

Nucleotide

• Length: 444 bp

>T211557 NZ_CP104927:24779-25261 [Klebsiella pasteurii]
TTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGAGAAACGGTACTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGGACGTTCGTTGTTTGCAACACGGGTACGA
AGCATGTAGCTGGTTTTTACTCTCTGGCCACTGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGAACCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCTCGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTCCACGCGCTTACTG
AAGAAGCCAAAGGCTTCTATGCTCACCATCGATTTATGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6886.64 Da
• Isoelectric Point: 4.6906

>AT211557 WP_003897042.1 NZ_CP080272:c5781717-5781529 [Mycolicibacterium smegmatis]
MGFLDKAKDLLSQNADKVDTVIDKAGDMIDEKTQGKYAQHVDKAQDAAKNAIRKDDPQEPQQ

Nucleotide

• Length: 189 bp

>AT211557 NZ_CP104927:24525-24791 [Klebsiella pasteurii]
ATGAAATCTGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACAGACTTCATTCTTGAGATGGCCTGCAAGGCCGCGGAGAACGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATTGATATGCTTGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCATTGGGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2U9PXD6