Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 4567906..4568447 Replicon chromosome
Accession NZ_CP080272
Organism Mycolicibacterium smegmatis strain Rabinowitchi

Toxin (Protein)


Gene name mazF Uniprot ID A0A2U9PVI3
Locus tag KZ782_RS21605 Protein ID WP_003895797.1
Coordinates 4568118..4568447 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0R0N3
Locus tag KZ782_RS21600 Protein ID WP_011729843.1
Coordinates 4567906..4568115 (+) Length 70 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ782_RS21575 (KZ782_21475) 4563177..4563947 - 771 WP_011729837.1 SDR family oxidoreductase -
KZ782_RS21580 4564484..4564885 - 402 Protein_4290 cupin domain-containing protein -
KZ782_RS21585 (KZ782_21485) 4564915..4565835 - 921 WP_233046304.1 NADP-dependent oxidoreductase -
KZ782_RS21590 (KZ782_21490) 4565832..4566116 - 285 WP_011729841.1 hypothetical protein -
KZ782_RS21595 (KZ782_21495) 4566312..4567745 + 1434 WP_233046718.1 dihydrolipoyl dehydrogenase -
KZ782_RS21600 (KZ782_21500) 4567906..4568115 + 210 WP_011729843.1 antitoxin MazE family protein Antitoxin
KZ782_RS21605 (KZ782_21505) 4568118..4568447 + 330 WP_003895797.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KZ782_RS21610 (KZ782_21510) 4568490..4569053 - 564 WP_233046719.1 helix-turn-helix domain-containing protein -
KZ782_RS21615 (KZ782_21515) 4569159..4570094 + 936 WP_003895799.1 alpha/beta fold hydrolase -
KZ782_RS21620 (KZ782_21520) 4570091..4571803 + 1713 WP_011729844.1 NAD(P)/FAD-dependent oxidoreductase -
KZ782_RS21630 (KZ782_21530) 4572020..4572400 + 381 WP_029104585.1 phage holin family protein -
KZ782_RS21635 (KZ782_21535) 4572418..4573443 + 1026 WP_014878040.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-104)

Antitoxin

Antitoxin MazE-like

(5-68)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 11739.52 Da        Isoelectric Point: 10.4023

>T211556 WP_003895797.1 NZ_CP080272:4568118-4568447 [Mycolicibacterium smegmatis]
VRRGDIYTAAARGAYTGKPRPVLIIQDDRFDATASVTVVPFTTSDTDAPLLRIRIEPTPATGLSTVSSLMIDKVTTVPRS
SLTHRVGRLAETDMVRTDRALLVFLGIAG

Download         Length: 330 bp

>T211556 NZ_CP104927:c3107-2811 [Klebsiella pasteurii]
GTGAAAACCGTTAAACTCACACCGAAAGCCAGTCAGGATCTGGAGGATATATGGTATTACGGGTACCACCATTTCGGTGA
AGAGCAGGCCGACAAATATATTAATCAGATATCCGACATTTTTCAGGTTGTGAGTGATCATAATATCGGTACTCCCCGTC
CTCAACTGGGGGAGCACATATGCGCATTACCCGTGGAACGGCATATGATTTATTTTCTGCAGACTGATACTGAAATTGTT
ATTATCCGTATATTAAGTCAGCATCAGGATGCCGGCCGTCATCTCAACTGGAAGTAA

Antitoxin


Download         Length: 70 a.a.        Molecular weight: 8114.98 Da        Isoelectric Point: 6.2231

>AT211556 WP_011729843.1 NZ_CP080272:4567906-4568115 [Mycolicibacterium smegmatis]
MTPARDRVRRHRERLRRQGLRPVQIWVPDVNAPEFRREAHRQSELVAAGEHEAEDQAFVDAISVDWDDA

Download         Length: 210 bp

>AT211556 NZ_CP104927:c3360-3094 [Klebsiella pasteurii]
ATGGCCAGAACAATGACAGTGAATCTCGGAGATGAACTCCGGAATTTCATAGAATCTCTCATAGAATCAGGTGATTATCG
TACACAGAGTGAAGTAATCCGCGACTCACTTCGCCTCCTGCGCGAGAAACAGGCAGAGTCACGTCTCCAGGCCCTGCGGG
ATTTACTGGCAGAAGGTCTGAGCAGCGGCGAACCTGTAACGTGGGAAAAAGATGCGTTTCTGAAAAAAGTGAAAGCTGGC
ATGAGGACTTCCGGTGAAAACCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U9PVI3

Antitoxin

Source ID Structure
AlphaFold DB A0R0N3

References