Detailed information of TA system TA211555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 3605830..3606826
• Replicon: chromosome
• Accession: NZ_CP080272
• Organism: Mycolicibacterium smegmatis strain Rabinowitchi

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: A0A2U9PRH2
• Locus tag: KZ782_RS17090
• Protein ID: WP_003894880.1
• Coordinates: 3606248..3606826 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: I7GB97
• Locus tag: KZ782_RS17085
• Protein ID: WP_003894879.1
• Coordinates: 3605830..3606222 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ782_RS17065 (KZ782_16990)	3601356..3602480	+	1125	WP_228025599.1	acyltransferase	-
KZ782_RS17070 (KZ782_16995)	3602592..3603197	+	606	WP_233045770.1	class I SAM-dependent methyltransferase	-
KZ782_RS17075 (KZ782_17000)	3603199..3604827	-	1629	WP_233045772.1	GMC family oxidoreductase N-terminal domain-containing protein	-
KZ782_RS17080 (KZ782_17005)	3605137..3605766	+	630	WP_003894878.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
KZ782_RS17085 (KZ782_17010)	3605830..3606222	+	393	WP_003894879.1	ParD-like family protein	Antitoxin
KZ782_RS17090 (KZ782_17015)	3606248..3606826	+	579	WP_003894880.1	zeta toxin family protein	Toxin
KZ782_RS17095 (KZ782_17020)	3606838..3608190	-	1353	WP_233045774.1	alpha/beta hydrolase-fold protein	-
KZ782_RS17100 (KZ782_17025)	3608524..3608679	+	156	WP_003894882.1	hypothetical protein	-
KZ782_RS17105 (KZ782_17030)	3608877..3610265	+	1389	WP_233045776.1	L-serine ammonia-lyase	-
KZ782_RS17110 (KZ782_17035)	3610299..3610859	-	561	WP_051065174.1	hypothetical protein	-
KZ782_RS17115 (KZ782_17040)	3611019..3611825	+	807	WP_233045778.1	transporter substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-136)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21119.30 Da
• Isoelectric Point: 9.5596

>T211555 WP_003894880.1 NZ_CP080272:3606248-3606826 [Mycolicibacterium smegmatis]
VKRLDLVVGPNGAGKSTFVELTLAPLLTGSAFVNADEIAKRRWPHAPAEHSYDAARIASDTRLKLIELGESFIAETVFSH
PSKIELIDTAQAAGYTVVMHVVMIPEDLAVLRVQHRVRAGGHHVPEEKIRERHQRLWPLVALAAARADISTFYDNSAVRG
PRIVAQLAGGFAVGRPAWPMWAPQVLTSRWPE

Nucleotide

• Length: 579 bp

>T211555 NZ_CP104926:c14266-13916 [Klebsiella pasteurii]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 13841.29 Da
• Isoelectric Point: 4.9246

>AT211555 WP_003894879.1 NZ_CP080272:3605830-3606222 [Mycolicibacterium smegmatis]
MTETADRVTRFAADLVESAAAEGARQSRSAKQQLDHWARVGRAVSSQHTAARRRVEAALAGDLALRDLTPEEGVVFNAEI
SAAIEENLAHTDYGQVLAGRGVTTVALDDDGAIVRYQPDGTTTVLAPAQR

Nucleotide

• Length: 393 bp

>AT211555 NZ_CP104926:c13911-13609 [Klebsiella pasteurii]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A2U9PRH2

Antitoxin

• AlphaFold DB: A0A2U9PRP8