Detailed information of TA system TA211553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 1437461..1438044
• Replicon: chromosome
• Accession: NZ_CP080272
• Organism: Mycolicibacterium smegmatis strain Rabinowitchi

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A2U9PKR4
• Locus tag: KZ782_RS06470
• Protein ID: WP_003892660.1
• Coordinates: 1437655..1438044 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: KZ782_RS06465
• Protein ID: WP_233044280.1
• Coordinates: 1437461..1437658 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ782_RS06445 (KZ782_06405)	1432508..1433518	-	1011	WP_233044274.1	AAA family ATPase	-
KZ782_RS06450 (KZ782_06410)	1433518..1434630	-	1113	WP_239738841.1	metallophosphoesterase	-
KZ782_RS06455 (KZ782_06415)	1434879..1436249	-	1371	WP_233044278.1	restriction endonuclease	-
KZ782_RS06460 (KZ782_06420)	1436406..1437431	-	1026	WP_233046807.1	HNH endonuclease domain-containing protein	-
KZ782_RS06465 (KZ782_06425)	1437461..1437658	+	198	WP_233044280.1	type II toxin-antitoxin system antitoxin Phd	Antitoxin
KZ782_RS06470 (KZ782_06430)	1437655..1438044	+	390	WP_003892660.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KZ782_RS06475 (KZ782_06435)	1438121..1439551	+	1431	WP_003892661.1	DUF3375 domain-containing protein	-
KZ782_RS06480 (KZ782_06440)	1439548..1440126	+	579	WP_038557632.1	DUF4194 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (18-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 13868.49 Da
• Isoelectric Point: 4.2481

>T211553 WP_003892660.1 NZ_CP080272:1437655-1438044 [Mycolicibacterium smegmatis]
VTEYLDREDVLTAGSIAFGGELKVRDYGLLDAAVARPQATVYGVDAYPRLWDKAAALLQSLARNHALVDGNKRTAWAAAW
TFLHINGVQLAADFDVDRAEDLMNEVATRDCDLDSIAAELAGFAAAQTG

Nucleotide

• Length: 390 bp

>T211553 NZ_CP104925:4773026-4773358 [Klebsiella pasteurii]
ATGAATACTTTACCTGCAATAAACCAGCGGGCTGTTCAGACATGCTTATCACCCGTGGTTGTCTGGCAAATGCTGCTTGC
TCGACTACTTGAGCAGCACTATGGCCTGACCTTAAATGACACGCCGTTCAGCGATGAGGCTGTTCTTAAAAGACATATCG
AAGCTGGTATCACGCTTGTCGATGCCGTTAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGATCGCAGAGGATTT
AGCTCACAGGGACAAGTGCCATATTTGACCGTGACAGATATCCTTCATGCCAGGAGAGAATGTGGATTAACGAACAGTTG
TCCTTATCGGTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7262.28 Da
• Isoelectric Point: 8.9352

>AT211553 WP_233044280.1 NZ_CP080272:1437461-1437658 [Mycolicibacterium smegmatis]
MPSLNIDFDEAEMEQIRAAARADDLSLKKFAHAAVMERASAHKRRVADAARLVAERSAELNRRLA

Nucleotide

• Length: 198 bp

>AT211553 NZ_CP104925:4772641-4772991 [Klebsiella pasteurii]
ATGTCGAGTAAGACACTCACAGTGAATGATGATACCGCTGAGCCCTGGTGGGGGCTGAATCGTAATGTCATTCCTTGTTT
TGGTGCGCGTCTGGTCCAGGAAGGTAACCGCCTATATTACCTGTCTGACCGCGCCAGCATAATAGGCCTATTCAATAAAG
CGGATTTGCTCCGTCTTGACCAGGCATTTCCTGTTCTGCTGAAGCAGGCGGAGCTCATGCTTACCAGCGGCGAACTGAAT
CCCCGACACCAGCATTGCGTCACCCTCTATGCAAAGGGGCTGACCTGCGAAGCTGATACACTGGGTTCCTGTGGTCACGT
CTATATCTCCATTTATCCTACTCAACGCTAA

Structures

Toxin

• AlphaFold DB: A0A2U9PKR4