Detailed information of TA system TA211551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2999194..2999828
• Replicon: chromosome
• Accession: NZ_CP080269
• Organism: Paenibacillus sp. R14(2021)

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KXU80_RS14030
• Protein ID: WP_090644310.1
• Coordinates: 2999194..2999544 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: KXU80_RS14035
• Protein ID: WP_090644307.1
• Coordinates: 2999547..2999828 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXU80_RS14005	2994408..2995856	-	1449	WP_219833893.1	ATPase, T2SS/T4P/T4SS family	-
KXU80_RS14010	2995853..2996713	-	861	WP_258171008.1	hypothetical protein	-
KXU80_RS14015	2996710..2997435	-	726	WP_219833894.1	SAF domain-containing protein	-
KXU80_RS14020	2997613..2998284	-	672	WP_219833895.1	hypothetical protein	-
KXU80_RS14025	2998303..2998998	-	696	WP_219833896.1	hypothetical protein	-
KXU80_RS14030	2999194..2999544	-	351	WP_090644310.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KXU80_RS14035	2999547..2999828	-	282	WP_090644307.1	ribbon-helix-helix protein, CopG family	Antitoxin
KXU80_RS14040	3000145..3001332	-	1188	WP_219833897.1	alanine racemase	-
KXU80_RS14045	3001600..3002940	-	1341	WP_219833898.1	outer membrane lipoprotein-sorting protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-46)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12836.81 Da
• Isoelectric Point: 5.1284

>T211551 WP_090644310.1 NZ_CP080269:c2999544-2999194 [Paenibacillus sp. R14(2021)]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAASHGFDRDSVLLLEQI
RTIDKQRLTDKITHLDDEMMRKVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T211551 NZ_CP104925:2173653-2173756 [Klebsiella pasteurii]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10593.22 Da
• Isoelectric Point: 7.0148

>AT211551 WP_090644307.1 NZ_CP080269:c2999828-2999547 [Paenibacillus sp. R14(2021)]
VANLQNTKRIMISLPDHLLEEVDGIVAKENSNRSEFIRQAMKLYLIERKKRQIRDSMQRGYLEMAKINLVMASEAFQAEE
DAGDTLGRLVSGV

Nucleotide

• Length: 282 bp

>AT211551 NZ_CP104925:c2173763-2173595 [Klebsiella pasteurii]
AATAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTTACCGTGTCAGGTTTTCCAGTCCGTGACAAAAGTG
GACGAATAA