Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 42027..42562 Replicon plasmid p135-3
Accession NZ_CP080263
Organism Escherichia coli strain 135

Toxin (Protein)


Gene name relE Uniprot ID V0VJJ7
Locus tag KZ312_RS27605 Protein ID WP_000222760.1
Coordinates 42275..42562 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag KZ312_RS27600 Protein ID WP_001132900.1
Coordinates 42027..42278 (+) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ312_RS27560 (37216) 37216..37428 + 213 WP_032303448.1 hypothetical protein -
KZ312_RS27565 (37674) 37674..38135 + 462 WP_059328048.1 thermonuclease family protein -
KZ312_RS27570 (38181) 38181..38390 + 210 WP_001299729.1 hemolysin expression modulator Hha -
KZ312_RS27575 (38429) 38429..38931 + 503 Protein_46 DUF2726 domain-containing protein -
- (39107) 39107..39168 - 62 NuclAT_1 - -
- (39107) 39107..39168 - 62 NuclAT_1 - -
- (39107) 39107..39168 - 62 NuclAT_1 - -
- (39107) 39107..39168 - 62 NuclAT_1 - -
KZ312_RS27580 (39212) 39212..39361 + 150 WP_001312851.1 Hok/Gef family protein -
KZ312_RS27585 (39645) 39645..39893 + 249 WP_000083837.1 replication regulatory protein RepA -
KZ312_RS27590 (40139) 40139..40213 + 75 WP_032144212.1 RepA leader peptide Tap -
KZ312_RS27595 (40206) 40206..41063 + 858 WP_029400224.1 incFII family plasmid replication initiator RepA -
KZ312_RS27600 (42027) 42027..42278 + 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KZ312_RS27605 (42275) 42275..42562 + 288 WP_000222760.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KZ312_RS29225 (42666) 42666..42986 + 321 Protein_53 serine acetyltransferase -
KZ312_RS27610 (43002) 43002..43349 - 348 Protein_54 IS1-like element IS1A family transposase -
KZ312_RS27615 (43438) 43438..44459 + 1022 Protein_55 IS21 family transposase -
KZ312_RS27620 (44456) 44456..45238 + 783 WP_001442137.1 IS21-like element IS100kyp family helper ATPase IstB -
KZ312_RS27625 (45293) 45293..45657 - 365 Protein_57 IS1 family transposase -
KZ312_RS27630 (45757) 45757..46960 + 1204 Protein_58 ISL3 family transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - papD / papC / papH 1..125082 125082


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-83)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11105.15 Da        Isoelectric Point: 10.5832

>T211549 WP_000222760.1 NZ_CP080263:42275-42562 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T211549 NZ_CP104925:884518-884928 [Klebsiella pasteurii]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGCGCGCAGTGGTTCTCACTGCTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGGTATTGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATATTGCTGTCGCTGGTAGTCTTCGACTGCG
TACGCAGCCAGCGGCGGATCCACGCCCGTCAGGGTGAAATAAAGCTGCTGATCGACTCGCGTCTGCGCTGGCAAAAGGCG
GAGTGGGATATCATCGGCACGCCGTGGGTAATCAACAGCGGCATGCTGCTGCGCCTGCGGAATACGGAAAACCAACGTAC
CCAGCATTTGTGGGTCGCGGCGGATAGCATGGACGCGGGAGAATGGCGCGACCTGCGTCGGCTAGTGTTACAGAAACCGA
CGCAAGAGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT211549 WP_001132900.1 NZ_CP080263:42027-42278 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT211549 NZ_CP104925:884271-884537 [Klebsiella pasteurii]
ATGGACATTAACAATAAAGCCCGCATCCACTGGGCATGTCGTCGAGGTATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTCGAGCATGAGTACGACACGCTAAGCGATGGCGATAAGGCGTTATTCATCCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGTTGATGAATCATGGCGAGCCGGCTGATGCTGAACTGCAGCGGATGGTAAAATTAATTCAGACTCGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0VJJ7


Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References