Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 113904..114429 | Replicon | plasmid p135-1 |
| Accession | NZ_CP080261 | ||
| Organism | Escherichia coli strain 135 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | S1PFV8 |
| Locus tag | KZ312_RS26425 | Protein ID | WP_001159871.1 |
| Coordinates | 114124..114429 (+) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | H9TJP1 |
| Locus tag | KZ312_RS26420 | Protein ID | WP_000813630.1 |
| Coordinates | 113904..114122 (+) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KZ312_RS26395 (KZ312_26395) | 108980..110208 | + | 1229 | WP_088895425.1 | IS3-like element IS2 family transposase | - |
| KZ312_RS26400 (KZ312_26400) | 110401..111608 | + | 1208 | WP_096990783.1 | IS3 family transposase | - |
| KZ312_RS26405 (KZ312_26405) | 112074..112526 | + | 453 | WP_000670963.1 | acyltransferase | - |
| KZ312_RS26410 (KZ312_26410) | 112558..112743 | - | 186 | WP_032353630.1 | hypothetical protein | - |
| KZ312_RS26415 (KZ312_26415) | 112791..112922 | - | 132 | Protein_125 | transposase | - |
| KZ312_RS26420 (KZ312_26420) | 113904..114122 | + | 219 | WP_000813630.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| KZ312_RS26425 (KZ312_26425) | 114124..114429 | + | 306 | WP_001159871.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| KZ312_RS26430 (KZ312_26430) | 114430..115239 | + | 810 | WP_045145707.1 | site-specific integrase | - |
| KZ312_RS26435 (KZ312_26435) | 115412..115765 | + | 354 | WP_000864812.1 | colicin M immunity protein | - |
| KZ312_RS26440 (KZ312_26440) | 115815..116630 | - | 816 | WP_000449473.1 | lipid II-degrading bacteriocin colicin M | - |
| KZ312_RS26445 (KZ312_26445) | 116872..117399 | + | 528 | WP_032353548.1 | colicin B immunity protein | - |
| KZ312_RS26450 (KZ312_26450) | 117417..118076 | - | 660 | Protein_132 | colicin-like pore-forming protein | - |
| KZ312_RS26455 (KZ312_26455) | 118137..118834 | + | 698 | WP_249940384.1 | IS1-like element IS1A family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | - | 1..135777 | 135777 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11692.49 Da Isoelectric Point: 6.4674
>T211545 WP_001159871.1 NZ_CP080261:114124-114429 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T211545 NZ_CP104920:3694661-3694918 [Dickeya solani]
ATGCCTGAGGTTGATAGACTGGCAGGGCTAATTTTCCATTTGTACTTTTATGATGATGAGCGGCACTACTTGCCGCATGT
ACATGTCTGCTACGGCAACCATGAGCTGATCATCAGTATTCGTACTGGAACCGAGATCGAAGGATATCTGCCGAATAACA
AGCGCAAGATGGCGCTTGCGCATATCAGGGCCCATCGGGAACAACTGCTGAGTATGTGGGAACGGGCGGTAGTGGGGAGA
AATCCAGGAAAATTGTGA
ATGCCTGAGGTTGATAGACTGGCAGGGCTAATTTTCCATTTGTACTTTTATGATGATGAGCGGCACTACTTGCCGCATGT
ACATGTCTGCTACGGCAACCATGAGCTGATCATCAGTATTCGTACTGGAACCGAGATCGAAGGATATCTGCCGAATAACA
AGCGCAAGATGGCGCTTGCGCATATCAGGGCCCATCGGGAACAACTGCTGAGTATGTGGGAACGGGCGGTAGTGGGGAGA
AATCCAGGAAAATTGTGA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8354.27 Da Isoelectric Point: 4.5203
>AT211545 WP_000813630.1 NZ_CP080261:113904-114122 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT211545 NZ_CP104920:3694926-3695441 [Dickeya solani]
ATGCATAAAATCGTGGAAGTTGACGTTATTGGTGATTTTTCGTTGCGCCTGACCTATGACGATGGTCTGGTTTGCGACAT
TAATCTTTATCAATATGCGTCCAGCCCAGTATTTTCGGACGTCAGCCTATTTATCAAGTTTGGGTTGTTGCCGGATGGCG
CGTTGGAATGGCTGCCGGATATTAAGATTTCCGCGTCAGAATTGCGGAAAAAAGGGGAGTATGTCAGCCACACGCTGGCG
GTGCGAGAAAGGCACTTCGCCGATGTCATCTCGCGCGCCTTCTATGATGCCGTGATTGAAAACCGGCCGGAAATTCTGCA
AGCAGCGTTGAAAATCGCGGTGGAAAAACATGGCAACAGCACCGTAGCCAAAGAGGCGGGGGCGAAAAGTCGCACCAGCT
TGTATAAGTCGCTGAATAAAAATACCCATGTCAGCCTGGCGACGGTGGTCAGCCTGGCGCATACCGTGCTGCAGTTGGAA
GAGTTGCAATTGGAAGATCTGCAATTGGAAGAATAA
ATGCATAAAATCGTGGAAGTTGACGTTATTGGTGATTTTTCGTTGCGCCTGACCTATGACGATGGTCTGGTTTGCGACAT
TAATCTTTATCAATATGCGTCCAGCCCAGTATTTTCGGACGTCAGCCTATTTATCAAGTTTGGGTTGTTGCCGGATGGCG
CGTTGGAATGGCTGCCGGATATTAAGATTTCCGCGTCAGAATTGCGGAAAAAAGGGGAGTATGTCAGCCACACGCTGGCG
GTGCGAGAAAGGCACTTCGCCGATGTCATCTCGCGCGCCTTCTATGATGCCGTGATTGAAAACCGGCCGGAAATTCTGCA
AGCAGCGTTGAAAATCGCGGTGGAAAAACATGGCAACAGCACCGTAGCCAAAGAGGCGGGGGCGAAAAGTCGCACCAGCT
TGTATAAGTCGCTGAATAAAAATACCCATGTCAGCCTGGCGACGGTGGTCAGCCTGGCGCATACCGTGCTGCAGTTGGAA
GAGTTGCAATTGGAAGATCTGCAATTGGAAGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CCE8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CEF5 |