211544

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 68323..68858 Replicon plasmid p135-1
Accession NZ_CP080261
Organism Escherichia coli strain 135

Toxin (Protein)


Gene name relE Uniprot ID A0A0D8WEY8
Locus tag KZ312_RS26170 Protein ID WP_032353555.1
Coordinates 68571..68858 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0D8WHE0
Locus tag KZ312_RS26165 Protein ID WP_045145550.1
Coordinates 68323..68574 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ312_RS29190 63833..64075 - 243 WP_021532691.1 PilI type IV pilus biogenesis protein -
KZ312_RS29195 64136..64309 - 174 Protein_71 hypothetical protein -
KZ312_RS26150 (KZ312_26150) 64352..65017 - 666 WP_058100831.1 traC -
KZ312_RS26155 (KZ312_26155) 65158..65666 - 509 Protein_73 transcription termination factor NusG -
KZ312_RS26160 (KZ312_26160) 66504..67376 + 873 WP_000729438.1 incFII family plasmid replication initiator RepA -
KZ312_RS26165 (KZ312_26165) 68323..68574 + 252 WP_045145550.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KZ312_RS26170 (KZ312_26170) 68571..68858 + 288 WP_032353555.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KZ312_RS26175 (KZ312_26175) 69138..69815 + 678 WP_072740705.1 IS66-like element accessory protein TnpA -
KZ312_RS26180 (KZ312_26180) 69815..70162 + 348 WP_000624618.1 IS66 family insertion sequence element accessory protein TnpB -
KZ312_RS26185 (KZ312_26185) 70182..71753 + 1572 WP_106487386.1 IS66 family transposase -
KZ312_RS26190 (KZ312_26190) 72364..72852 + 489 WP_072157196.1 serine acetyltransferase -
KZ312_RS26195 (KZ312_26195) 73132..73725 - 594 WP_032353553.1 ProQ/FINO family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..135777 135777


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11114.16 Da        Isoelectric Point: 10.6261

>T211544 WP_032353555.1 NZ_CP080261:68571-68858 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKAIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR

Download         Length: 288 bp

>T211544 NZ_CP104920:c3242300-3242097 [Dickeya solani]
ATGAAAAAAATCGACTATTTAATGCGTTTGCGTAAATGCACAACCATCGACACGCTTGAGCGTGTTATAGAAAAAAACAA
GTACGAGCTTTCCAACGATGAGCTTGAAATGTTCTATTCAGCTGCAGACCACCGCCTGGCAGAGCTGACGATGAATAAGC
TTTACGACAAAGTACCGACTGCTGTGTGGAGATACGTTCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9219.48 Da        Isoelectric Point: 4.1789

>AT211544 WP_045145550.1 NZ_CP080261:68323-68574 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHYVDLD
SYL

Download         Length: 252 bp

>AT211544 NZ_CP104920:c3242722-3242354 [Dickeya solani]
ATGGATGAGTACACACCCCAACATTATGATATTGCCCAACTCAGATTCTTGTGTGAAAATCTGCACGACGAAAGTATCGC
GACATTAGGTGACAGCAGTCATGGCTGGGTGAATGATCCGACCTCTGCGGTCAACCTTCAACTAAACGAACTTATCGAGC
ATATTGCGGCATTTGTTGTTACCTACAAAATAAAATACCCGCATGAAGCGGCGCTATGTGAACGGGTTGAGAAATATCTG
GACGACACTTATATTCTTTTCAGCAATTACGGTATCAATGACACCGAGTTGCGGAAATGGCAAAAATCCAAGTCACAATT
ATTCAGAATGTTCTCAGAAAAGAGCATCTGTACGGTAGTAAAAACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8WEY8

Antitoxin

Source ID Structure
AlphaFold DB A0A0D8WHE0

References