Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4594339..4594993 Replicon chromosome
Accession NZ_CP080260
Organism Escherichia coli strain 135

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag KZ312_RS22000 Protein ID WP_000244781.1
Coordinates 4594339..4594746 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KZ312_RS22005 Protein ID WP_000354046.1
Coordinates 4594727..4594993 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ312_RS21980 (4590296) 4590296..4592029 - 1734 WP_000813175.1 single-stranded-DNA-specific exonuclease RecJ -
KZ312_RS21985 (4592035) 4592035..4592745 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KZ312_RS21990 (4592770) 4592770..4593666 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KZ312_RS21995 (4593778) 4593778..4594299 + 522 WP_001055888.1 flavodoxin FldB -
KZ312_RS22000 (4594339) 4594339..4594746 - 408 WP_000244781.1 protein YgfX Toxin
KZ312_RS22005 (4594727) 4594727..4594993 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KZ312_RS22010 (4595236) 4595236..4596216 + 981 WP_000886050.1 tRNA-modifying protein YgfZ -
KZ312_RS22015 (4596293) 4596293..4596952 - 660 WP_000250269.1 hemolysin III family protein -
KZ312_RS22020 (4597116) 4597116..4597427 - 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
KZ312_RS22025 (4597472) 4597472..4598905 + 1434 WP_059327701.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T211541 WP_000244781.1 NZ_CP080260:c4594746-4594339 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T211541 NZ_CP104920:2763980-2764411 [Dickeya solani]
GTGGCCCAGTGGCAATGTGATTTGCGCGTATCCTGGCGCATGCAGCTGTTTTCGCTGCTGACGCACGGGTTTCTGGTGCT
GATGATCCTGCTGGCGCCCTGGCCGGACGGCTATGCGCCGCTGTGGCTGGGGTTGGTGACGCTGGTGGTCTTCGGTTTCG
TGCGCAGTCAGCGCATCATCAAGTCGCGTCAGGGAGAAATTTCCCTGCATGGGGAAAACCAACTGCACTGGCAGCAGCGC
GACTGGCAGATAGCCCGGCGGCCGTGGGTGATGCGCAACGGTGTGTTGCTGTCATTGCGGGCGACGAATGGCAAAGGACA
CCAACGCCTGTGGCTGGCGTCAGACAGTATGGGCAATGAAGAATGGCGGCTGTTGCGCCAGCTGTTGCAGCAGCATCCGC
TGCAAGGTACGGAATCCTCCCGCCATCATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT211541 WP_000354046.1 NZ_CP080260:c4594993-4594727 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT211541 NZ_CP104920:2763733-2763999 [Dickeya solani]
ATGGACATCAATAACAAATCACGTATTCACTGGGCGTGCAGACGCGGAATGCGTGAACTGGATATCTCCATCATGCCGTT
TTTCGAGCATGAGTACGATACGTTGAGCGACGACGACAAGCAGCAGTTCATCCGTCTGCTGCAGTGCGACGATCCCGACT
TGTTCAACTGGTTGATGAACCATGGCGAACCGGTGGATCCGGCGCTGAAACGTATGGTTTCCCTTATTCAGACGCGAAAT
AAAGACCGTGGCCCAGTGGCAATGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References