Detailed information of TA system TA021154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 3483752..3484354
• Replicon: chromosome
• Accession: NC_006513
• Organism: Aromatoleum aromaticum EbN1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: EB_RS16305
• Protein ID: WP_011239114.1
• Coordinates: 3483752..3484042 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: EB_RS16310
• Protein ID: WP_041646526.1
• Coordinates: 3484052..3484354 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB_RS16270	3479032..3479301	+	270	WP_011239109.1	30S ribosomal protein S15	-
EB_RS16275	3479464..3481563	+	2100	WP_011239110.1	polyribonucleotide nucleotidyltransferase	-
EB_RS16280	3481649..3482074	-	426	WP_011239111.1	RNA polymerase-binding protein DksA	-
EB_RS16300	3482620..3483474	-	855	WP_157866635.1	hypothetical protein	-
EB_RS16305	3483752..3484042	+	291	WP_011239114.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EB_RS16310	3484052..3484354	+	303	WP_041646526.1	putative addiction module antidote protein	Antitoxin
EB_RS24340	3484955..3485113	+	159	WP_157866636.1	hypothetical protein	-
EB_RS16315	3485155..3486555	+	1401	WP_011239116.1	putative DNA binding domain-containing protein	-
EB_RS16320	3486558..3488012	+	1455	WP_011239117.1	MBL fold metallo-hydrolase	-
EB_RS16325	3488260..3488790	+	531	WP_197531910.1	nucleotidyltransferase domain-containing protein	-
EB_RS16330	3488780..3489217	+	438	WP_011239119.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-90)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10721.38 Da
• Isoelectric Point: 7.9987

>T21154 WP_011239114.1 NC_006513:3483752-3484042 [Aromatoleum aromaticum EbN1]
MKTVLTTEFFDAWFAELRDPQAAARIKMRIGRAELGNFGDCEPVGEGVSEMRIHYGPGYRLYFTQRGLEVVILLAGGDKS
SQAKDIKTALKLAREL

Nucleotide

• Length: 291 bp

>T21154 NC_006513:3483752-3484042 [Aromatoleum aromaticum EbN1]
ATGAAGACTGTGCTCACCACCGAATTCTTCGATGCCTGGTTTGCCGAGCTCCGCGACCCGCAGGCGGCAGCCCGCATCAA
GATGCGAATCGGTCGGGCAGAGTTGGGCAACTTCGGCGACTGCGAACCGGTGGGCGAGGGGGTTTCGGAGATGCGGATCC
ATTACGGGCCGGGCTACCGCCTGTATTTCACGCAGCGCGGCCTGGAAGTGGTGATCCTGCTGGCGGGCGGCGACAAGTCC
TCCCAGGCCAAGGACATCAAGACCGCCTTGAAGCTGGCCCGGGAACTGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10777.43 Da
• Isoelectric Point: 6.7188

>AT21154 WP_041646526.1 NC_006513:3484052-3484354 [Aromatoleum aromaticum EbN1]
MALKLRKWDTAEHLKTEEEMALYLEACLEEAGDDAAFIAKALGDIARAKGMTQLAKDTGLGRESLYKALSGEGNPSFATI
LKVTRALGLQLHAAPRHAPV

Nucleotide

• Length: 303 bp

>AT21154 NC_006513:3484052-3484354 [Aromatoleum aromaticum EbN1]
ATGGCACTCAAATTGCGCAAATGGGACACGGCAGAGCACCTCAAGACCGAGGAGGAGATGGCGCTCTACCTCGAGGCTTG
TCTGGAAGAAGCCGGGGACGATGCAGCCTTTATCGCGAAGGCACTGGGCGATATTGCCCGCGCCAAAGGCATGACCCAAC
TGGCCAAGGACACCGGTCTGGGGCGGGAGAGCCTGTACAAGGCCCTTTCGGGCGAGGGCAATCCGAGCTTTGCCACCATC
CTCAAGGTGACACGCGCGTTGGGCCTGCAACTGCACGCGGCACCGCGCCACGCGCCGGTGTGA