Detailed information of TA system TA021153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Gp49-Ata
• Location: 2223403..2224004
• Replicon: chromosome
• Accession: NC_006513
• Organism: Aromatoleum aromaticum EbN1

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: EB_RS10385
• Protein ID: WP_041647153.1
• Coordinates: 2223684..2224004 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: EB_RS10380
• Protein ID: WP_011237941.1
• Coordinates: 2223403..2223687 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB_RS10375	2221719..2223098	-	1380	WP_011236947.1	IS110-like element ISAzo22 family transposase	-
EB_RS10380	2223403..2223687	-	285	WP_011237941.1	helix-turn-helix domain-containing protein	Antitoxin
EB_RS10385	2223684..2224004	-	321	WP_041647153.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EB_RS23300	2224169..2224591	+	423	WP_157866715.1	hypothetical protein	-
EB_RS24230	2224715..2224930	+	216	WP_157866606.1	hypothetical protein	-
EB_RS10400	2224930..2225313	+	384	WP_041646194.1	type II toxin-antitoxin system VapC family toxin	-
EB_RS10405	2225317..2225721	-	405	WP_041646196.1	transcriptional regulator	-
EB_RS24240	2225998..2226165	+	168	WP_197531808.1	hypothetical protein	-
EB_RS10410	2226432..2227952	+	1521	WP_011235976.1	IS21-like element ISAzo12 family transposase	-
EB_RS10415	2227965..2228753	+	789	WP_011235977.1	IS21-like element ISAzo12 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2221719..2228753	7034

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-93)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12144.06 Da
• Isoelectric Point: 10.0558

>T21153 WP_041647153.1 NC_006513:c2224004-2223684 [Aromatoleum aromaticum EbN1]
MKTLRFVGSSLDDLQDFPADARRAAGFELFAVQRGLMPTDFKPMLAVGPGAYEIRLHVLGEWRVIYVARFEDAVYVLHAF
QKKTQKTPKQDIELAARRYKQIGDRT

Nucleotide

• Length: 321 bp

>T21153 NC_006513:c2224004-2223684 [Aromatoleum aromaticum EbN1]
ATGAAGACGCTACGCTTCGTCGGTTCGAGCCTCGACGATCTGCAGGATTTCCCCGCCGACGCTCGCAGGGCAGCCGGCTT
CGAGCTGTTTGCAGTGCAGCGCGGACTGATGCCTACCGACTTCAAGCCAATGCTGGCCGTCGGCCCGGGCGCTTACGAAA
TACGGCTGCACGTTCTCGGCGAGTGGCGCGTGATCTACGTCGCCAGGTTTGAAGATGCGGTATACGTACTGCACGCTTTC
CAGAAGAAGACGCAGAAGACCCCGAAGCAGGATATCGAGCTGGCGGCACGACGGTACAAGCAGATCGGAGACCGGACATG
A

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10241.79 Da
• Isoelectric Point: 6.3296

>AT21153 WP_011237941.1 NC_006513:c2223687-2223403 [Aromatoleum aromaticum EbN1]
MTKEAIVESSGNVFADLGFSPEEAALLAMRAELIARLRETIEQAGWTQVQAASRLGIGQSRVSDLMRGKYDKFSLDMLVT
LATRAGRRVELAVA

Nucleotide

• Length: 285 bp

>AT21153 NC_006513:c2223687-2223403 [Aromatoleum aromaticum EbN1]
ATGACCAAGGAAGCAATCGTCGAATCGAGTGGCAATGTGTTTGCCGATCTGGGGTTCTCGCCCGAAGAAGCGGCGCTGCT
GGCGATGCGCGCCGAATTGATCGCACGTCTGCGCGAAACCATCGAGCAGGCGGGATGGACGCAGGTACAGGCGGCAAGCC
GGCTGGGTATCGGGCAGTCGCGCGTGTCGGACCTGATGCGTGGCAAGTACGACAAGTTCAGCCTCGATATGCTGGTCACG
CTCGCCACGCGGGCCGGCCGGCGGGTCGAGCTTGCGGTCGCCTGA