Detailed information of TA system TA211528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2928460..2929098
• Replicon: chromosome
• Accession: NZ_CP080260
• Organism: Escherichia coli strain 135

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D3GRG9
• Locus tag: KZ312_RS14090
• Protein ID: WP_001314712.1
• Coordinates: 2928460..2928636 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KZ312_RS14095
• Protein ID: WP_077248684.1
• Coordinates: 2928682..2929098 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ312_RS14070 (2924082)	2924082..2925293	-	1212	WP_086795292.1	BenE family transporter YdcO	-
KZ312_RS14075 (2925346)	2925346..2925882	+	537	WP_000429149.1	DNA-binding transcriptional regulator SutR	-
KZ312_RS14080 (2925955)	2925955..2927916	+	1962	WP_059327963.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KZ312_RS14085 (2928008)	2928008..2928238	-	231	WP_072262409.1	YncJ family protein	-
KZ312_RS14090 (2928460)	2928460..2928636	+	177	WP_001314712.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KZ312_RS14095 (2928682)	2928682..2929098	+	417	WP_077248684.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KZ312_RS14100 (2929177)	2929177..2930583	+	1407	WP_000760623.1	PLP-dependent aminotransferase family protein	-
KZ312_RS14105 (2930828)	2930828..2931973	+	1146	WP_000047443.1	ABC transporter substrate-binding protein	-
KZ312_RS14110 (2931991)	2931991..2933004	+	1014	WP_000220407.1	ABC transporter ATP-binding protein	-
KZ312_RS14115 (2933005)	2933005..2933946	+	942	WP_086795293.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (87-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6746.81 Da
• Isoelectric Point: 11.8888

>T211528 WP_001314712.1 NZ_CP080260:2928460-2928636 [Escherichia coli]
VKQSEFRRWLESQGVNVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T211528 NZ_CP104914:1911398-1911682 [Avibacterium paragallinarum]
ATGAGCTATGAGCTAGTTTTTGATCCAAGGGCGTTAAAAGAATGGCGTAAACTTGGTGAAAATATAAAAGCACAATTTAA
GAAAAAATTGGCTGAGATTTTGCGTAATCCTCGTATAGAAGCCAATCGGCTTAGTCATTTTCCTGATTGCTATAAGATAA
AACTACGCAATGCAGGATACCGTTTGATTTATCAAGTTCAAGATGAGCAAGTGGTTGTTTTTGTTGTGGCTATCGGTAAA
CGAGAAAACAACCAAGCTTACAAAGAGGCAAAAACGAGGCTATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15232.60 Da
• Isoelectric Point: 4.7286

>AT211528 WP_077248684.1 NZ_CP080260:2928682-2929098 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSDITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT211528 NZ_CP104914:1911166-1911408 [Avibacterium paragallinarum]
ATGGCAACACTTAATATTCGGCTTGATGATGAACTAAAGCAAGAAGCTTATGCGGCATTACAGAAAATGAACCTGACACC
AAGTGAAGCGGTAAGATTATTATTCCAATATGTAGCACAAAATAATAAATTTCCTATTCAAACCGTGTTAGTCAATGATG
AAGATCTTGAACTGCTAAAAACGGTACGTCATCGCCTAGCCCACCCCGAAAAAGGCATTAAGGTTAGCTTAGATGAGCTA
TGA

Structures

Toxin

• AlphaFold DB: D3GRG9