Detailed information of TA system TA211519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2424791..2425575
• Replicon: chromosome
• Accession: NZ_CP080260
• Organism: Escherichia coli strain 135

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: KZ312_RS11430
• Protein ID: WP_000613626.1
• Coordinates: 2424791..2425285 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: KZ312_RS11435
• Protein ID: WP_001110447.1
• Coordinates: 2425282..2425575 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ312_RS11410 (2419984)	2419984..2421081	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
KZ312_RS11415 (2421081)	2421081..2422022	+	942	WP_001586904.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
KZ312_RS11420 (2422088)	2422088..2423731	+	1644	WP_086795335.1	flagellar hook-associated protein FlgK	-
KZ312_RS11425 (2423743)	2423743..2424696	+	954	WP_001586905.1	flagellar hook-associated protein FlgL	-
KZ312_RS11430 (2424791)	2424791..2425285	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
KZ312_RS11435 (2425282)	2425282..2425575	-	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
KZ312_RS11440 (2425708)	2425708..2429007	-	3300	WP_203397158.1	ribonuclease E	-
KZ312_RS11445 (2429580)	2429580..2430539	+	960	WP_001586906.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T211519 WP_000613626.1 NZ_CP080260:c2425285-2424791 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T211519 NZ_CP104913:c143848-143567 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGAGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT211519 WP_001110447.1 NZ_CP080260:c2425575-2425282 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT211519 NZ_CP104913:c144072-143845 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGACCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6