Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1140514..1141064 Replicon chromosome
Accession NZ_CP080260
Organism Escherichia coli strain 135

Toxin (Protein)


Gene name ccdB Uniprot ID A0A244BU83
Locus tag KZ312_RS05305 Protein ID WP_001160962.1
Coordinates 1140750..1141064 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag KZ312_RS05300 Protein ID WP_000125566.1
Coordinates 1140514..1140747 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ312_RS05275 (1135587) 1135587..1135874 + 288 WP_086795132.1 ferredoxin-like protein FixX -
KZ312_RS05280 (1135933) 1135933..1137264 + 1332 WP_001183198.1 MFS transporter -
KZ312_RS05285 (1137372) 1137372..1137902 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
KZ312_RS05290 (1137895) 1137895..1139757 + 1863 WP_000377114.1 glutathione-regulated potassium-efflux system protein KefC -
KZ312_RS05295 (1139949) 1139949..1140428 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
KZ312_RS05300 (1140514) 1140514..1140747 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
KZ312_RS05305 (1140750) 1140750..1141064 + 315 WP_001160962.1 CcdB family protein Toxin
KZ312_RS05310 (1141061) 1141061..1141909 - 849 WP_021556397.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
KZ312_RS05315 (1141916) 1141916..1142293 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
KZ312_RS05320 (1142296) 1142296..1143117 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
KZ312_RS05325 (1143114) 1143114..1144103 - 990 WP_000241280.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
KZ312_RS05330 (1144103) 1144103..1145389 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11741.79 Da        Isoelectric Point: 9.0851

>T211513 WP_001160962.1 NZ_CP080260:1140750-1141064 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFIRIRPPERLNTILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T211513 NZ_CP104907:255667-256056 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT211513 WP_000125566.1 NZ_CP080260:1140514-1140747 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT211513 NZ_CP104907:255403-255660 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A244BU83

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References