Detailed information of TA system TA021151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1992106..1992776
• Replicon: chromosome
• Accession: NC_006513
• Organism: Aromatoleum aromaticum EbN1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q5P3U6
• Locus tag: EB_RS09280
• Protein ID: WP_011237711.1
• Coordinates: 1992357..1992776 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EB_RS09275
• Protein ID: WP_041647107.1
• Coordinates: 1992106..1992360 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB_RS09250	1988016..1988495	-	480	Protein_1839	ISAs1-like element ISAzo29 family transposase	-
EB_RS09260	1988812..1990381	+	1570	Protein_1840	IS3-like element ISAzo10 family transposase	-
EB_RS24220	1990475..1991233	+	759	WP_157866604.1	hypothetical protein	-
EB_RS09265	1991331..1991657	-	327	WP_173361273.1	type II toxin-antitoxin system PemK/MazF family toxin	-
EB_RS09270	1991654..1991878	-	225	WP_011237709.1	antitoxin MazE family protein	-
EB_RS09275	1992106..1992360	+	255	WP_041647107.1	Arc family DNA-binding protein	Antitoxin
EB_RS09280	1992357..1992776	+	420	WP_011237711.1	type II toxin-antitoxin system VapC family toxin	Toxin
EB_RS09285	1992854..1993204	+	351	WP_011237713.1	hypothetical protein	-
EB_RS09290	1993214..1993630	+	417	WP_011237714.1	hypothetical protein	-
EB_RS09295	1993792..1994622	-	831	WP_011237715.1	hypothetical protein	-
EB_RS09300	1994950..1995528	-	579	WP_011237716.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-120)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14858.29 Da
• Isoelectric Point: 6.9803

>T21151 WP_011237711.1 NC_006513:1992357-1992776 [Aromatoleum aromaticum EbN1]
MILLDTNVVSEPLRHAPEVRVIEWIDVQPLETLFLSTITVAELRAGVALLPAGKRRSGLQENLEKRVLPLFAGRVLPFDL
ACTRAYAELMAKARAAGLAIATADGYIAAIAAANGFTVATRYTSPFEAAGAAVINPWQA

Nucleotide

• Length: 420 bp

>T21151 NC_006513:1992357-1992776 [Aromatoleum aromaticum EbN1]
ATGATCCTGCTGGACACCAATGTGGTGTCGGAGCCGCTTCGCCACGCGCCCGAAGTCCGCGTGATCGAGTGGATCGATGT
CCAGCCGCTGGAAACGCTTTTTTTGTCCACCATCACCGTCGCGGAATTGCGCGCCGGGGTGGCGCTGCTGCCTGCTGGCA
AGCGGCGCTCGGGCTTGCAGGAAAACCTGGAAAAGCGCGTGCTGCCGCTGTTCGCCGGCCGCGTGCTGCCCTTTGACCTG
GCGTGTACGCGAGCCTATGCGGAGCTGATGGCCAAGGCACGAGCAGCCGGGCTCGCCATTGCCACCGCTGACGGCTATAT
CGCCGCAATCGCTGCCGCCAACGGTTTCACGGTCGCCACGCGGTACACCAGTCCCTTCGAGGCCGCCGGCGCAGCGGTCA
TCAACCCTTGGCAGGCATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9484.85 Da
• Isoelectric Point: 7.2067

>AT21151 WP_041647107.1 NC_006513:1992106-1992360 [Aromatoleum aromaticum EbN1]
MAMLTVRNLPEEVHRALRVRAAQHGRSMEAEVREILESVVSPQGRVKLGSLLADLGRQARLSDEEFAVFEQVRDKTPSRP
VSFE

Nucleotide

• Length: 255 bp

>AT21151 NC_006513:1992106-1992360 [Aromatoleum aromaticum EbN1]
ATGGCCATGTTGACCGTCCGCAATCTTCCGGAGGAGGTGCACCGGGCCCTGCGCGTTCGTGCCGCCCAGCATGGCCGCAG
CATGGAGGCCGAGGTGCGTGAGATTCTGGAGTCCGTCGTCAGCCCGCAAGGCCGCGTGAAGCTGGGCTCGCTGCTGGCCG
ACTTGGGCCGCCAGGCCAGGCTGAGCGATGAGGAGTTTGCCGTTTTCGAGCAGGTGCGTGACAAGACTCCTTCTCGCCCG
GTGAGCTTCGAATGA

Structures

Toxin

• AlphaFold DB: Q5P3U6