Detailed information of TA system TA021150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1991331..1991878
• Replicon: chromosome
• Accession: NC_006513
• Organism: Aromatoleum aromaticum EbN1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EB_RS09265
• Protein ID: WP_173361273.1
• Coordinates: 1991331..1991657 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q5P3U8
• Locus tag: EB_RS09270
• Protein ID: WP_011237709.1
• Coordinates: 1991654..1991878 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB_RS09245	1987075..1987809	+	735	WP_011236010.1	IS21-like element ISAzo4 family helper ATPase IstB	-
EB_RS09250	1988016..1988495	-	480	Protein_1839	ISAs1-like element ISAzo29 family transposase	-
EB_RS09260	1988812..1990381	+	1570	Protein_1840	IS3-like element ISAzo10 family transposase	-
EB_RS24220	1990475..1991233	+	759	WP_157866604.1	hypothetical protein	-
EB_RS09265	1991331..1991657	-	327	WP_173361273.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EB_RS09270	1991654..1991878	-	225	WP_011237709.1	antitoxin MazE family protein	Antitoxin
EB_RS09275	1992106..1992360	+	255	WP_041647107.1	Arc family DNA-binding protein	-
EB_RS09280	1992357..1992776	+	420	WP_011237711.1	type II toxin-antitoxin system VapC family toxin	-
EB_RS09285	1992854..1993204	+	351	WP_011237713.1	hypothetical protein	-
EB_RS09290	1993214..1993630	+	417	WP_011237714.1	hypothetical protein	-
EB_RS09295	1993792..1994622	-	831	WP_011237715.1	hypothetical protein	-
EB_RS09300	1994950..1995528	-	579	WP_011237716.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• Antitoxin MazE-like (8-72)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11598.63 Da
• Isoelectric Point: 9.2533

>T21150 WP_173361273.1 NC_006513:c1991657-1991331 [Aromatoleum aromaticum EbN1]
VMRGDFVTIVMQGDFGKPRPALVIQADSFSEHATVTVLPVTSTLVAAPLFRITVQPSPENGLQKPSQVMADKAMTVKRAK
IGQAFGRIDADAMVEVERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T21150 NC_006513:c1991657-1991331 [Aromatoleum aromaticum EbN1]
GTGATGCGGGGCGACTTCGTGACCATCGTCATGCAAGGCGACTTTGGCAAGCCAAGACCCGCCCTGGTGATTCAAGCCGA
CTCGTTCAGCGAGCACGCCACCGTCACGGTGCTGCCGGTAACAAGCACGCTGGTCGCTGCGCCGCTGTTTCGCATCACTG
TCCAACCCAGCCCGGAGAACGGCCTGCAGAAGCCTTCGCAGGTGATGGCGGACAAGGCCATGACCGTGAAACGCGCCAAG
ATAGGGCAAGCCTTCGGCCGCATTGATGCAGATGCGATGGTGGAGGTCGAGCGTTGCCTGGCCGTGTTTCTGGGGATCGC
CAAGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8579.56 Da
• Isoelectric Point: 5.6833

>AT21150 WP_011237709.1 NC_006513:c1991878-1991654 [Aromatoleum aromaticum EbN1]
MANTPVNARVQKHRDARRRAGLRPVQIWVPDTRRPDFAEECRRQSRLAAEADMADTDMQHFMDEALADVDGWTE

Nucleotide

• Length: 225 bp

>AT21150 NC_006513:c1991878-1991654 [Aromatoleum aromaticum EbN1]
ATGGCAAACACGCCTGTAAATGCACGGGTTCAAAAGCACCGTGACGCGCGGCGCAGGGCCGGGCTTCGCCCGGTGCAAAT
CTGGGTGCCGGATACGCGACGGCCAGACTTTGCAGAGGAATGTCGCCGCCAGAGCCGCCTTGCTGCCGAAGCGGACATGG
CCGACACGGACATGCAGCACTTCATGGATGAAGCCCTGGCAGACGTTGACGGCTGGACGGAGTGA

Structures

Antitoxin

• AlphaFold DB: Q5P3U8