Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4343880..4344377 Replicon chromosome
Accession NZ_CP080255
Organism Escherichia coli strain SJC33

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KZ313_RS20645 Protein ID WP_001173343.1
Coordinates 4343880..4344053 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KZ313_RS20650 Protein ID WP_000398619.1
Coordinates 4344081..4344377 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ313_RS20635 (4340550) 4340550..4342007 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
KZ313_RS20640 (4342244) 4342244..4343761 + 1518 WP_001550472.1 lysine--tRNA ligase -
KZ313_RS20645 (4343880) 4343880..4344053 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KZ313_RS20650 (4344081) 4344081..4344377 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KZ313_RS20655 (4344606) 4344606..4344878 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KZ313_RS20660 (4344890) 4344890..4345120 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
KZ313_RS20665 (4345300) 4345300..4346931 + 1632 WP_001216477.1 sensor histidine kinase -
KZ313_RS20670 (4346928) 4346928..4347647 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T211496 WP_001173343.1 NZ_CP080255:c4344053-4343880 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T211496 NZ_CP104882:c11874-11524 [Aggregatibacter actinomycetemcomitans]
ATGTGGGCGGTGATTTTAACGGAACGATTTGAAACTTGGTTAAATCAACAGCCGATAAAAACGCAGGAATGTGTTTTGGC
AAACTTGGTACGGTTGGAACATTTTGGTCCGAATTTAGCCCGTCCTTATGTAGATAGCGTAGCAGGGTCAATTCATAGCA
ACATGAAAGAACTACGCGTTCAGCATTCGGGAAGAGCAATTCGACTCTTTTTTGCTTTTGATCCAAAGCGTAGAGCCATT
GTTTTATGTGGTGGAGATAAAGGCAATGACAAGCGGTTTTACCGTACGATGATTAGAATTGCAGATCAAGAATTGAATCA
TTATTTGGCAACCATGGAGCAAACAAAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT211496 WP_000398619.1 NZ_CP080255:c4344377-4344081 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT211496 NZ_CP104882:c11527-11228 [Aggregatibacter actinomycetemcomitans]
ATGAAAACATTACAACAAGTTATTGATGAACGTTCGGTAGAAAGCCGCCAACGTATTCAAACCATGGCGGAAGATTTAAT
TCTTGAAACCGGTTTGCAACTGTTGCGAGAAGAATTAAATATATCGCAACAACAATTAGCTACGATTTTAGGTGTAACTC
AACCTGCAATTAATCAACTGGAACAGCGTGGCAATGAAATTAAGCTTGCAACCCTCAAACGTTACATCGAAGCAATGGGC
GGCAAATTACGCCTTAGTGTAGAAATGCCTGATGGACATCAGCGGGTTTTTCAAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References