Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3562527..3563145 Replicon chromosome
Accession NZ_CP080255
Organism Escherichia coli strain SJC33

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KZ313_RS16980 Protein ID WP_001291435.1
Coordinates 3562927..3563145 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KZ313_RS16975 Protein ID WP_000344800.1
Coordinates 3562527..3562901 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ313_RS16965 (3557616) 3557616..3558809 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KZ313_RS16970 (3558832) 3558832..3561981 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
KZ313_RS16975 (3562527) 3562527..3562901 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KZ313_RS16980 (3562927) 3562927..3563145 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KZ313_RS16985 (3563317) 3563317..3563868 + 552 WP_000102564.1 maltose O-acetyltransferase -
KZ313_RS16990 (3563984) 3563984..3564454 + 471 WP_000136192.1 YlaC family protein -
KZ313_RS16995 (3564618) 3564618..3566168 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KZ313_RS17000 (3566210) 3566210..3566563 - 354 WP_001550741.1 DUF1428 family protein -
KZ313_RS17010 (3566942) 3566942..3567253 + 312 WP_000409911.1 MGMT family protein -
KZ313_RS17015 (3567284) 3567284..3567856 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T211493 WP_001291435.1 NZ_CP080255:3562927-3563145 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T211493 NZ_CP104878:1836458-1836634 [Bacillus subtilis]
GTGCTTGAGAAAGTGGGTATCATAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATAAACAGTCTAACAATAGTTGA
GAAGGTAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT211493 WP_000344800.1 NZ_CP080255:3562527-3562901 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT211493 NZ_CP104878:c1836675-1836575 [Bacillus subtilis]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTGCGTATACGCTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References