Detailed information of TA system TA211490

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2572317..2572955
• Replicon: chromosome
• Accession: NZ_CP080255
• Organism: Escherichia coli strain SJC33

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: KZ313_RS12410
• Protein ID: WP_000813795.1
• Coordinates: 2572779..2572955 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KZ313_RS12405
• Protein ID: WP_076797675.1
• Coordinates: 2572317..2572733 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ313_RS12385 (2567469)	2567469..2568410	-	942	WP_001251315.1	ABC transporter permease	-
KZ313_RS12390 (2568411)	2568411..2569424	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
KZ313_RS12395 (2569442)	2569442..2570587	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
KZ313_RS12400 (2570832)	2570832..2572238	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
KZ313_RS12405 (2572317)	2572317..2572733	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KZ313_RS12410 (2572779)	2572779..2572955	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KZ313_RS12415 (2573177)	2573177..2573407	+	231	WP_023910283.1	YncJ family protein	-
KZ313_RS12420 (2573499)	2573499..2575460	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KZ313_RS12425 (2575533)	2575533..2576069	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
KZ313_RS12430 (2576122)	2576122..2577333	+	1212	WP_071591517.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T211490 WP_000813795.1 NZ_CP080255:c2572955-2572779 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T211490 NZ_CP104878:519580-519930 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT211490 WP_076797675.1 NZ_CP080255:c2572733-2572317 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT211490 NZ_CP104878:519294-519575 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1