Detailed information of TA system TA211489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2473010..2474598
• Replicon: chromosome
• Accession: NZ_CP080255
• Organism: Escherichia coli strain SJC33

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A829LC11
• Locus tag: KZ313_RS12000
• Protein ID: WP_001551128.1
• Coordinates: 2473276..2474598 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KZ313_RS11995
• Protein ID: WP_001296726.1
• Coordinates: 2473010..2473276 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ313_RS11995 (2473010)	2473010..2473276	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KZ313_RS12000 (2473276)	2473276..2474598	+	1323	WP_001551128.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
KZ313_RS12005 (2474798)	2474798..2476150	-	1353	WP_001551127.1	SIR2 family protein	-
KZ313_RS12010 (2476365)	2476365..2477397	-	1033	Protein_2353	ISNCY family transposase	-
KZ313_RS12015 (2477688)	2477688..2477948	+	261	Protein_2354	aldo/keto reductase	-
KZ313_RS12020 (2478094)	2478094..2478789	+	696	WP_001552076.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2476365..2477183	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49270.88 Da
• Isoelectric Point: 8.2472

>T211489 WP_001551128.1 NZ_CP080255:2473276-2474598 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T211489 NZ_CP104871:c355339-354815 [Pseudomonas aeruginosa]
ATGAAAATCATCGGAGTGCAGGACCTCTCCTGCTACCGAATGCACACTCCCAAGTGGTCCGTTGCGCCTACATCTGGAGC
TGGAGCTGCAACTCAGGGTGGCCGGGTTAACCGAAAGGGGCTAGAGGCTCTATACCTCTCGCTTGAGTCGGCAACGGCCA
TTGCTGAGTTTCAGCAAGCAAGCGCATTCCTTCCACCTGGTCTCCTGGTCAGCTATTCCCTCAGTCTGGATCGAGTGATC
GACTTCACCGGCGGCTATAACGATACCTGGGCCCCGATCTGGCAAGACTTCTTCTGCGACTGGAGAAAAATTCGCTTCAA
CGACGGCGTTGAACCGCCGAGCTGGATTATCGGGGATCTGGCTATCGAGGCTGACTGTGCTGGGATTCTGTTCGAGTCCG
TTGCGAACCCTGGAGGTCGGAACCTGGTGCTTTTCACCGATCAGTTGCCGGTGCATGGGAATATTGTGGTCAACGATCCT
CGCGGGGATCTCCCTACCGACCAGTCATCTTGGACGCGCCCGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT211489 WP_001296726.1 NZ_CP080255:2473010-2473276 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT211489 NZ_CP104871:c355731-355336 [Pseudomonas aeruginosa]
ATGGCCAGCAAAGCCGTATTAGAGCTTCGCCCCACCAACTTCGCGGGCTTGATGGACGCTCTCAAAGAGGTGAACCGTGT
GATGTTCTCTCCTGAGCGCTTCCTCAAGGTTTTTTCGATGGACCAGCAGACCTTGGCGCTCCGGGCCCATGTCCATCGCA
ACACTGTGCGCAACGCGCCTGAATCGGAAAAGGTCCAGGCCTACATTCGTGATTCAGTGAAGGTTCTGCGCGCAGTGACC
GACATGGGCACGGACGTGACGAATGCCATCTTCTGGTTTAAGAACGAGCCTCTTTCCACGTTCGGCTATAAAACCGCGGA
GGAAGTGGTCAGCGAAGGAAAAACCGAGCAGCTGATCGCTTTCCTTCAATCCTGGGAAGCTGGTGCCCAAGGATGA

Structures

Toxin

• AlphaFold DB: A0A829LC11