Detailed information of TA system TA021146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/GNAT-DUF1778
• Location: 1191423..1192201
• Replicon: chromosome
• Accession: NC_006513
• Organism: Aromatoleum aromaticum EbN1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q5P616
• Locus tag: EB_RS05650
• Protein ID: WP_011236966.1
• Coordinates: 1191698..1192201 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: EB_RS05645
• Protein ID: WP_041645866.1
• Coordinates: 1191423..1191701 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB_RS05620	1187641..1188375	+	735	WP_011235973.1	IS21-like element ISAzo4 family helper ATPase IstB	-
EB_RS05625	1188579..1188992	+	414	Protein_1110	IS21 family transposase	-
EB_RS05630	1188989..1189768	+	780	WP_011236051.1	IS21-like element ISAzo17 family helper ATPase IstB	-
EB_RS05635	1189965..1190246	-	282	WP_011236962.1	DUF1778 domain-containing protein	-
EB_RS05640	1190370..1190975	-	606	WP_041645864.1	EAL domain-containing protein	-
EB_RS05645	1191423..1191701	+	279	WP_041645866.1	DUF1778 domain-containing protein	Antitoxin
EB_RS05650	1191698..1192201	+	504	WP_011236966.1	GNAT family N-acetyltransferase	Toxin
EB_RS05655	1192308..1193141	-	834	WP_157866583.1	NAD(+) diphosphatase	-
EB_RS05660	1193226..1194620	-	1395	WP_011236968.1	heavy metal sensor histidine kinase	-
EB_RS05665	1194617..1195297	-	681	WP_011236969.1	heavy metal response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-88)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18171.82 Da
• Isoelectric Point: 7.3497

>T21146 WP_011236966.1 NC_006513:1191698-1192201 [Aromatoleum aromaticum EbN1]
MSAAYEPISKLGAADAVESFDCGQPALNQFLHQYALVNQKANSAQTYLCRSAGEVVGFYSLAVGSVDPESAPARVMKGLA
RHPVPVMILARLAVDKNHQGKGLGQALLKDALQRTAQAADIAGIRCLLVHAKDNAARQWYESWEFEPSQTDSYHLFLMLK
DLKALLT

Nucleotide

• Length: 504 bp

>T21146 NC_006513:1191698-1192201 [Aromatoleum aromaticum EbN1]
TTGAGCGCTGCGTATGAGCCGATAAGCAAACTCGGTGCCGCAGACGCCGTCGAAAGTTTCGACTGTGGCCAGCCGGCACT
GAATCAGTTTCTGCACCAGTACGCACTGGTCAACCAGAAGGCCAATAGCGCACAAACCTACCTCTGCCGCAGTGCCGGTG
AGGTCGTCGGTTTCTACAGCCTCGCGGTAGGCAGCGTTGATCCCGAGTCGGCACCTGCGCGGGTGATGAAAGGCCTCGCT
CGACACCCTGTCCCGGTGATGATTCTGGCAAGGCTGGCGGTGGACAAAAATCATCAGGGCAAAGGGCTGGGTCAAGCGCT
GCTCAAGGACGCCCTGCAGCGCACCGCCCAGGCGGCCGACATCGCGGGCATTCGATGCTTGCTGGTGCACGCGAAGGACA
ACGCGGCGCGGCAGTGGTACGAGTCCTGGGAGTTCGAGCCGAGCCAGACCGACTCTTATCATTTATTCTTGATGCTCAAG
GACTTGAAGGCTTTGTTAACGTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10354.85 Da
• Isoelectric Point: 10.0968

>AT21146 WP_041645866.1 NC_006513:1191423-1191701 [Aromatoleum aromaticum EbN1]
MTTLNQSKTERIDVRASGPVKQLLQEAARACHKNVSEFLLDAGVTAANQALADRRRFVLDDERWQAFQQALDRPVQAKPR
LKKLLTEPGVLD

Nucleotide

• Length: 279 bp

>AT21146 NC_006513:1191423-1191701 [Aromatoleum aromaticum EbN1]
ATGACCACCTTGAACCAATCGAAAACCGAACGGATCGATGTCCGGGCGAGCGGCCCCGTCAAGCAGCTCCTGCAGGAAGC
AGCGCGTGCTTGCCACAAGAACGTCAGCGAGTTCCTGCTCGACGCTGGCGTGACTGCGGCTAACCAGGCGCTGGCCGACC
GTCGCCGTTTCGTTCTCGACGACGAGCGGTGGCAGGCATTCCAGCAGGCACTGGATCGCCCGGTGCAAGCCAAACCGCGC
CTGAAGAAACTGCTCACCGAACCTGGAGTGCTGGATTGA

Structures

Toxin

• AlphaFold DB: Q5P616