Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 844902..845556 Replicon chromosome
Accession NZ_CP080253
Organism Escherichia coli strain PC6

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KZX77_RS04105 Protein ID WP_000244777.1
Coordinates 845149..845556 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KZX77_RS04100 Protein ID WP_000354046.1
Coordinates 844902..845168 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX77_RS04075 (840071) 840071..840814 + 744 WP_000951964.1 SDR family oxidoreductase -
KZX77_RS04080 (840871) 840871..842304 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
KZX77_RS04085 (842349) 842349..842660 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
KZX77_RS04090 (842824) 842824..843483 + 660 WP_000250274.1 hemolysin III family protein -
KZX77_RS04095 (843679) 843679..844659 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KZX77_RS04100 (844902) 844902..845168 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KZX77_RS04105 (845149) 845149..845556 + 408 WP_000244777.1 protein YgfX Toxin
KZX77_RS04110 (845596) 845596..846117 - 522 WP_001055874.1 flavodoxin FldB -
KZX77_RS04115 (846229) 846229..847125 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KZX77_RS04120 (847150) 847150..847860 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KZX77_RS04125 (847866) 847866..849599 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T211453 WP_000244777.1 NZ_CP080253:845149-845556 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T211453 NZ_CP104866:2704793-2705251 [Pseudomonas aeruginosa]
ATGCAACGGCATGGCTGGACGCTGCTCTTCCACGACTGCGTGATCGAGCAGTTGCAGAAACTGCATGCGGCCGCGAGGCG
CGCGCAGGAGAATGACCCGGCGGGCTTCGAGTCCAATGCCAACGTCAAGCTCTTCCGGGCCCTGAGCCAGTTGATGTTGG
ATGTGGTGCCAGGCGATCCGGCACGCGACGAGTACCGCCAGGGCAACACCTTGGGACCTGCCCACCGCCACTGGCGAAGG
GCCAAGATCGGACGGCGGTTCCGGCTGTTCTTCCGGTACGACTCGAAGGCGAAGGTCATTGTGTACGCCTGGGTCAACGA
TGAACAGACCCTGCGGTCTTCGGGTAGCAAATCGGACCCGTATGTCGTGTTCGAGAAGATGCTCGGACGCGGGAACCCAC
CAGACGACTGGCACGCGCTGATACAGGCAAGCAAGCAGGATTGGAGCAAACTGGAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT211453 WP_000354046.1 NZ_CP080253:844902-845168 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT211453 NZ_CP104866:2704476-2704793 [Pseudomonas aeruginosa]
ATGCCTGCCATCCACGAAGTCGCCACGCTGACCTCTAAAGGCCAGATCACGCTGCCCAAGCCCATCCGGCAGGCGCTCGG
CGTCGATGCCGGCGGCAAGCTCGCGTTCGATCTGCGGGGCAGCGAAGTCGTCGTCACCCGTGTTGATGCCGAGCACGAGG
ACCCCGCCATTGGCGCGTTTCTGAGCCTGCTGGCCCGCGACATCGAAGCTGGCCGGAACGTCCGGGGCCTGCCCGAGGAT
CTGGCGCGCGCCATGCTGGAGCATGCTGGCCACGGTGTGAACCTGGACGAGGAGATCGACGGGAAAGTGGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References