Detailed information of TA system TA211424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2245183..2245712
• Replicon: chromosome
• Accession: NZ_CP080249
• Organism: Staphylococcus aureus strain NV_1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KZW86_RS11370
• Protein ID: WP_000621175.1
• Coordinates: 2245183..2245545 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KZW86_RS11375
• Protein ID: WP_000948331.1
• Coordinates: 2245542..2245712 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW86_RS11340 (2240719)	2240719..2242038	+	1320	WP_001557163.1	ISL3-like element IS1181 family transposase	-
KZW86_RS11345 (2242041)	2242041..2242112	+	72	Protein_2195	hypothetical protein	-
KZW86_RS11350 (2242161)	2242161..2242931	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
KZW86_RS11355 (2242906)	2242906..2243385	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
KZW86_RS11360 (2243387)	2243387..2243713	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KZW86_RS11365 (2243832)	2243832..2244833	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KZW86_RS11370 (2245183)	2245183..2245545	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KZW86_RS11375 (2245542)	2245542..2245712	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KZW86_RS11380 (2245797)	2245797..2246945	-	1149	WP_001281154.1	alanine racemase	-
KZW86_RS11385 (2247011)	2247011..2247370	-	360	WP_000581197.1	holo-ACP synthase	-
KZW86_RS11390 (2247374)	2247374..2247865	-	492	WP_001205912.1	PH domain-containing protein	-
KZW86_RS11395 (2247852)	2247852..2249435	-	1584	WP_001294626.1	PH domain-containing protein	-
KZW86_RS11400 (2249428)	2249428..2249907	-	480	WP_001287087.1	hypothetical protein	-
KZW86_RS11405 (2250115)	2250115..2250675	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2240719..2242038	1319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T211424 WP_000621175.1 NZ_CP080249:c2245545-2245183 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T211424 NZ_CP104858:2350314-2350598 [Salmonella sp. 3C]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGATTAA
GAAAAAGTTGGCTGATGTGCTATTGGACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGAAGTTGTGATTGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT211424 WP_000948331.1 NZ_CP080249:c2245712-2245542 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT211424 NZ_CP104858:2350082-2350324 [Salmonella sp. 3C]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1