Detailed information of TA system TA021142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 914240..914850
• Replicon: chromosome
• Accession: NC_006513
• Organism: Aromatoleum aromaticum EbN1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EB_RS04340
• Protein ID: WP_041645754.1
• Coordinates: 914240..914419 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EB_RS04345
• Protein ID: WP_041645757.1
• Coordinates: 914431..914850 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB_RS04305	910243..910500	+	258	WP_011236694.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
EB_RS04310	910511..910822	+	312	WP_197531874.1	Txe/YoeB family addiction module toxin	-
EB_RS04315	910794..912371	-	1578	WP_011236060.1	IS66-like element ISAzo15 family transposase	-
EB_RS04320	912468..912806	-	339	WP_011236696.1	IS66 family insertion sequence element accessory protein TnpB	-
EB_RS04325	912806..913132	-	327	WP_041645449.1	IS66 family insertion sequence hypothetical protein	-
EB_RS04330	913431..913751	-	321	WP_041645748.1	hypothetical protein	-
EB_RS04335	913738..914019	-	282	WP_041645751.1	hypothetical protein	-
EB_RS04340	914240..914419	+	180	WP_041645754.1	type II toxin-antitoxin system HicA family toxin	Toxin
EB_RS04345	914431..914850	+	420	WP_041645757.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EB_RS04350	915181..916272	+	1092	WP_011236701.1	hypothetical protein	-
EB_RS04355	916324..916569	+	246	WP_041645761.1	DUF4160 domain-containing protein	-
EB_RS04360	916607..917644	+	1038	WP_011236702.1	IS5-like element ISAzo11 family transposase	-
EB_RS04365	917834..918088	+	255	WP_011236703.1	DUF2442 domain-containing protein	-
EB_RS04370	918532..918810	+	279	WP_011236705.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EB_RS04375	918820..919137	+	318	WP_011236706.1	HigA family addiction module antidote protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	910794..917644	6850

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-64)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6434.55 Da
• Isoelectric Point: 11.2470

>T21142 WP_041645754.1 NC_006513:914240-914419 [Aromatoleum aromaticum EbN1]
MNSKQMKKWLEQQGATFAPGKGSHLKVFLNGRQSSLPMHGTAELGKGLEAAIKRQLGLK

Nucleotide

• Length: 180 bp

>T21142 NC_006513:914240-914419 [Aromatoleum aromaticum EbN1]
ATGAACTCGAAGCAGATGAAGAAATGGCTTGAGCAGCAGGGTGCGACATTTGCGCCTGGCAAGGGCTCACATCTGAAGGT
GTTCTTGAACGGTCGCCAGTCCTCACTTCCTATGCACGGTACGGCTGAGTTGGGCAAGGGGCTGGAAGCGGCGATCAAAC
GTCAGTTGGGCTTGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15257.35 Da
• Isoelectric Point: 4.7532

>AT21142 WP_041645757.1 NC_006513:914431-914850 [Aromatoleum aromaticum EbN1]
MFDYPVVLTPDENGTVLVTFPDVPEAITFGADEDEALLLAVDALETGLSFYVDARKPLPTPSSAQGRPTVRPSALECAKL
GVYQAMTEQGLRKTDLARRLGWHLPQVDRLFDLSHASRFDQLEAAARALGRHIEVRVAP

Nucleotide

• Length: 420 bp

>AT21142 NC_006513:914431-914850 [Aromatoleum aromaticum EbN1]
ATGTTCGATTACCCCGTAGTCCTGACACCCGACGAGAATGGCACGGTGCTGGTGACGTTTCCCGATGTGCCCGAAGCCAT
CACCTTTGGCGCAGATGAGGATGAGGCGCTGTTGCTGGCGGTGGATGCGCTGGAGACCGGGCTGTCGTTCTATGTCGATG
CGCGTAAGCCACTGCCGACGCCTTCCTCTGCGCAGGGCCGCCCAACGGTGCGGCCTTCCGCGCTGGAGTGTGCCAAGCTC
GGGGTGTATCAAGCGATGACCGAGCAGGGCTTGCGCAAGACGGATCTGGCACGTCGTCTGGGCTGGCATCTGCCTCAGGT
GGATCGCCTGTTTGATCTTTCTCACGCTTCACGCTTCGATCAACTCGAAGCCGCCGCCCGTGCGCTGGGTCGGCATATCG
AGGTGCGTGTTGCCCCGTAG