Detailed information of TA system TA021140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 386082..386749
• Replicon: chromosome
• Accession: NC_006513
• Organism: Aromatoleum aromaticum EbN1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EB_RS01750
• Protein ID: WP_197531869.1
• Coordinates: 386318..386749 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q5P894
• Locus tag: EB_RS01745
• Protein ID: WP_011236200.1
• Coordinates: 386082..386312 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB_RS01735	381935..383836	+	1902	WP_011236198.1	DNA mismatch repair endonuclease MutL	-
EB_RS01740	383937..385913	+	1977	WP_011236199.1	type IIA DNA topoisomerase subunit B	-
EB_RS01745	386082..386312	+	231	WP_011236200.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EB_RS01750	386318..386749	+	432	WP_197531869.1	type II toxin-antitoxin system VapC family toxin	Toxin
EB_RS01755	386746..389106	+	2361	WP_011236202.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-121)

Antitoxin

• SpoVT-AbrB domain (7-42)

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15970.25 Da
• Isoelectric Point: 6.3260

>T21140 WP_197531869.1 NC_006513:386318-386749 [Aromatoleum aromaticum EbN1]
MLDTNICIYLINHRPAHVRAHFDGHPIGDIGVSVITAAELAYGVTKSASPRNRAALETFLLPLEVAPFDDRAIWRYAELR
TQLMRRGTPIGALDTQIAAHALALDCTLVTNNTREFERVDGLRLENWVDQRLMESPAAYFTAP

Nucleotide

• Length: 432 bp

>T21140 NC_006513:386318-386749 [Aromatoleum aromaticum EbN1]
ATGCTCGATACCAACATCTGCATCTACCTCATCAATCATCGCCCAGCCCACGTGCGCGCCCACTTCGATGGGCACCCGAT
CGGCGACATCGGCGTATCGGTCATCACCGCGGCGGAACTCGCGTATGGCGTAACGAAAAGCGCTTCACCCCGCAACCGTG
CAGCGCTGGAAACCTTTCTGCTGCCGCTGGAGGTGGCCCCTTTTGACGACAGGGCGATCTGGCGCTATGCCGAACTGCGT
ACGCAACTGATGCGACGCGGCACGCCGATCGGCGCGCTCGACACCCAGATTGCGGCGCATGCGCTTGCGCTCGACTGCAC
GCTGGTCACCAACAACACACGCGAATTCGAACGGGTGGACGGCCTGCGCCTGGAAAACTGGGTCGACCAGCGCCTGATGG
AATCCCCTGCCGCTTACTTCACCGCTCCATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8487.69 Da
• Isoelectric Point: 5.0931

>AT21140 WP_011236200.1 NC_006513:386082-386312 [Aromatoleum aromaticum EbN1]
MDTAKLFTNGRSQAVRLPKEFRFEGDEVFIKRVGDAVVLLPRHGWGTLFGALEAFEPGFTIERGAPEQAEREALLP

Nucleotide

• Length: 231 bp

>AT21140 NC_006513:386082-386312 [Aromatoleum aromaticum EbN1]
ATGGATACCGCCAAACTTTTCACGAACGGCCGCAGCCAGGCGGTACGCCTGCCCAAGGAGTTCCGCTTCGAGGGCGACGA
AGTGTTCATCAAGCGCGTCGGCGACGCCGTCGTGCTGCTGCCGCGCCACGGCTGGGGTACGCTGTTCGGGGCGCTGGAGG
CCTTCGAGCCGGGATTCACGATCGAGCGCGGCGCGCCGGAACAAGCGGAACGCGAGGCGCTGTTGCCGTGA

Structures

Antitoxin

• AlphaFold DB: Q5P894