Detailed information of TA system TA211398

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3558047..3558726
• Replicon: chromosome
• Accession: NZ_CP080247
• Organism: Escherichia sp. TM-G17TGC

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: KY274_RS17375
• Protein ID: WP_000854672.1
• Coordinates: 3558385..3558726 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: KY274_RS17370
• Protein ID: WP_000070395.1
• Coordinates: 3558047..3558364 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KY274_RS17320 (3553094)	3553094..3553957	+	864	WP_001065553.1	GTPase family protein	-
KY274_RS17325 (3554049)	3554049..3554870	+	822	WP_000197389.1	DUF932 domain-containing protein	-
KY274_RS17330 (3555087)	3555087..3555278	+	192	Protein_3392	DeoR family transcriptional regulator	-
KY274_RS17335 (3555325)	3555325..3555501	+	177	WP_001285112.1	hypothetical protein	-
KY274_RS17340 (3555501)	3555501..3555944	+	444	WP_000824223.1	lipoprotein YafY	-
KY274_RS17345 (3555967)	3555967..3556434	+	468	WP_001547765.1	protein YkfB	-
KY274_RS17350 (3556511)	3556511..3556750	+	240	WP_000194654.1	DUF905 family protein	-
KY274_RS17355 (3556848)	3556848..3557306	+	459	WP_000211838.1	antirestriction protein	-
KY274_RS17360 (3557322)	3557322..3557798	+	477	WP_000811693.1	RadC family protein	-
KY274_RS17365 (3557807)	3557807..3558028	+	222	WP_000691994.1	DUF987 domain-containing protein	-
KY274_RS17370 (3558047)	3558047..3558364	+	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
KY274_RS17375 (3558385)	3558385..3558726	+	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
KY274_RS17385 (3559298)	3559298..3560551	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
KY274_RS17390 (3560563)	3560563..3561666	-	1104	WP_001285288.1	glutamate 5-kinase	-
KY274_RS17395 (3561954)	3561954..3562313	+	360	Protein_3404	porin	-
KY274_RS17400 (3562420)	3562420..3563628	+	1209	WP_001339175.1	IS4-like element ISVsa5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T211398 WP_000854672.1 NZ_CP080247:3558385-3558726 [Escherichia sp. TM-G17TGC]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T211398 NZ_CP104851:3605044-3605262 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT211398 WP_000070395.1 NZ_CP080247:3558047-3558364 [Escherichia sp. TM-G17TGC]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT211398 NZ_CP104851:3604644-3605018 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684