Detailed information of TA system TA211396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2368461..2369099
• Replicon: chromosome
• Accession: NZ_CP080247
• Organism: Escherichia sp. TM-G17TGC

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KY274_RS11465
• Protein ID: WP_000813794.1
• Coordinates: 2368923..2369099 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KY274_RS11460
• Protein ID: WP_001270286.1
• Coordinates: 2368461..2368877 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KY274_RS11440 (2363613)	2363613..2364554	-	942	WP_001251304.1	ABC transporter permease	-
KY274_RS11445 (2364555)	2364555..2365568	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
KY274_RS11450 (2365586)	2365586..2366731	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
KY274_RS11455 (2366976)	2366976..2368382	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
KY274_RS11460 (2368461)	2368461..2368877	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KY274_RS11465 (2368923)	2368923..2369099	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KY274_RS11470 (2369321)	2369321..2369551	+	231	WP_000494244.1	YncJ family protein	-
KY274_RS11475 (2369643)	2369643..2371604	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KY274_RS11480 (2371677)	2371677..2372213	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
KY274_RS11485 (2372305)	2372305..2373480	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T211396 WP_000813794.1 NZ_CP080247:c2369099-2368923 [Escherichia sp. TM-G17TGC]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T211396 NZ_CP104851:c2900197-2899814 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT211396 WP_001270286.1 NZ_CP080247:c2368877-2368461 [Escherichia sp. TM-G17TGC]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT211396 NZ_CP104851:c2900658-2900278 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Toxin

• PDB: 6HPB