Detailed information of TA system TA211385

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1800019..1800850
• Replicon: chromosome
• Accession: NZ_CP080247
• Organism: Escherichia sp. TM-G17TGC

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: KY274_RS08560
• Protein ID: WP_000854814.1
• Coordinates: 1800019..1800393 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: KY274_RS08565
• Protein ID: WP_001285584.1
• Coordinates: 1800482..1800850 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KY274_RS08520 (1795415)	1795415..1796581	+	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
KY274_RS08525 (1796700)	1796700..1797173	+	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
KY274_RS08530 (1797371)	1797371..1798429	+	1059	WP_046613327.1	FUSC family protein	-
KY274_RS08535 (1798601)	1798601..1798930	+	330	WP_000450409.1	DUF496 family protein	-
KY274_RS08540 (1799031)	1799031..1799165	-	135	WP_001297944.1	EutP/PduV family microcompartment system protein	-
KY274_RS08545 (1799285)	1799285..1799413	+	129	Protein_1670	transposase domain-containing protein	-
KY274_RS08550 (1799702)	1799702..1799782	-	81	Protein_1671	hypothetical protein	-
KY274_RS08555 (1799828)	1799828..1800022	-	195	WP_000988600.1	DUF5983 family protein	-
KY274_RS08560 (1800019)	1800019..1800393	-	375	WP_000854814.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
KY274_RS08565 (1800482)	1800482..1800850	-	369	WP_001285584.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
KY274_RS08570 (1800924)	1800924..1801145	-	222	WP_000692323.1	DUF987 domain-containing protein	-
KY274_RS08575 (1801208)	1801208..1801654	-	447	WP_000187523.1	RadC family protein	-
KY274_RS08580 (1801651)	1801651..1803183	-	1533	WP_001350525.1	protein YeeR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T211385 WP_000854814.1 NZ_CP080247:c1800393-1800019 [Escherichia sp. TM-G17TGC]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T211385 NZ_CP104851:903826-904233 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT211385 WP_001285584.1 NZ_CP080247:c1800850-1800482 [Escherichia sp. TM-G17TGC]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT211385 NZ_CP104851:903579-903845 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• PDB: 2H28
• AlphaFold DB: A0A1M2E8G6