Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4183144..4183694 Replicon chromosome
Accession NZ_CP080246
Organism Escherichia coli strain SYNB1934

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag KZX95_RS20095 Protein ID WP_001160966.1
Coordinates 4183380..4183694 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag KZX95_RS20090 Protein ID WP_000125566.1
Coordinates 4183144..4183377 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX95_RS20065 (4178217) 4178217..4178504 + 288 WP_000203741.1 ferredoxin-like protein FixX -
KZX95_RS20070 (4178563) 4178563..4179894 + 1332 WP_001183207.1 MFS transporter -
KZX95_RS20075 (4180002) 4180002..4180532 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
KZX95_RS20080 (4180525) 4180525..4182387 + 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
KZX95_RS20085 (4182579) 4182579..4183058 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
KZX95_RS20090 (4183144) 4183144..4183377 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
KZX95_RS20095 (4183380) 4183380..4183694 + 315 WP_001160966.1 CcdB family protein Toxin
KZX95_RS20100 (4183691) 4183691..4184539 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
KZX95_RS20105 (4184546) 4184546..4184923 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
KZX95_RS20110 (4184926) 4184926..4185747 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
KZX95_RS20115 (4185744) 4185744..4186733 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
KZX95_RS20120 (4186733) 4186733..4188019 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T211372 WP_001160966.1 NZ_CP080246:4183380-4183694 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T211372 NZ_CP104848:c3317661-3317407 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT211372 WP_000125566.1 NZ_CP080246:4183144-4183377 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT211372 NZ_CP104848:c3317909-3317658 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References