Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3994259..3995094 Replicon chromosome
Accession NZ_CP080246
Organism Escherichia coli strain SYNB1934

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0J2AEA6
Locus tag KZX95_RS19170 Protein ID WP_000854759.1
Coordinates 3994717..3995094 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag KZX95_RS19165 Protein ID WP_001295723.1
Coordinates 3994259..3994627 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX95_RS19140 (3991374) 3991374..3991569 + 196 Protein_3752 DUF905 family protein -
KZX95_RS19145 (3991687) 3991687..3992505 + 819 WP_001234738.1 DUF932 domain-containing protein -
KZX95_RS19150 (3992847) 3992847..3993320 + 474 WP_001350782.1 antirestriction protein -
KZX95_RS19155 (3993336) 3993336..3993812 + 477 WP_001186775.1 RadC family protein -
KZX95_RS19160 (3993875) 3993875..3994096 + 222 WP_000692345.1 DUF987 domain-containing protein -
KZX95_RS19165 (3994259) 3994259..3994627 + 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KZX95_RS19170 (3994717) 3994717..3995094 + 378 WP_000854759.1 TA system toxin CbtA family protein Toxin
KZX95_RS19175 (3995091) 3995091..3995579 + 489 WP_000761690.1 DUF5983 family protein -
KZX95_RS19180 (3995596) 3995596..3995772 + 177 WP_000839286.1 DUF957 domain-containing protein -
KZX95_RS19185 (3995878) 3995878..3996027 + 150 Protein_3761 hypothetical protein -
KZX95_RS19190 (3996394) 3996394..3996570 + 177 Protein_3762 helix-turn-helix domain-containing protein -
KZX95_RS19195 (3997361) 3997361..3998983 + 1623 WP_001295726.1 Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3980801..4009244 28443


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14144.26 Da        Isoelectric Point: 7.3249

>T211368 WP_000854759.1 NZ_CP080246:3994717-3995094 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T211368 NZ_CP104848:c2455846-2455739 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT211368 WP_001295723.1 NZ_CP080246:3994259-3994627 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT211368 NZ_CP104848:2455894-2455960 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2AEA6

Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References