Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2861365..2862166 Replicon chromosome
Accession NZ_CP080246
Organism Escherichia coli strain SYNB1934

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0H2V687
Locus tag KZX95_RS13455 Protein ID WP_000854739.1
Coordinates 2861786..2862166 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0P7R0L9
Locus tag KZX95_RS13450 Protein ID WP_001285482.1
Coordinates 2861365..2861739 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX95_RS13415 (2857453) 2857453..2857908 + 456 WP_000581502.1 IrmA family protein -
KZX95_RS13420 (2858051) 2858051..2858140 + 90 WP_230607454.1 DUF905 family protein -
KZX95_RS13425 (2858319) 2858319..2859140 + 822 WP_001234710.1 DUF932 domain-containing protein -
KZX95_RS13430 (2859403) 2859403..2859876 + 474 WP_000855076.1 antirestriction protein -
KZX95_RS13435 (2859892) 2859892..2860368 + 477 WP_001186170.1 RadC family protein -
KZX95_RS13440 (2860431) 2860431..2860652 + 222 WP_000692312.1 DUF987 domain-containing protein -
KZX95_RS13445 (2860671) 2860671..2861315 + 645 WP_000094915.1 hypothetical protein -
KZX95_RS13450 (2861365) 2861365..2861739 + 375 WP_001285482.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KZX95_RS13455 (2861786) 2861786..2862166 + 381 WP_000854739.1 TA system toxin CbtA family protein Toxin
KZX95_RS13460 (2862163) 2862163..2862651 + 489 WP_001054232.1 DUF5983 family protein -
KZX95_RS13465 (2862671) 2862671..2862868 + 198 WP_000772027.1 DUF957 domain-containing protein -
KZX95_RS13470 (2862953) 2862953..2863796 + 844 Protein_2637 DUF4942 domain-containing protein -
KZX95_RS13480 (2864267) 2864267..2865205 + 939 WP_000351287.1 glyoxylate/hydroxypyruvate reductase GhrA -
KZX95_RS13485 (2865260) 2865260..2865997 + 738 WP_000283664.1 zinc-binding phosphatase -
KZX95_RS13490 (2866021) 2866021..2866575 + 555 WP_001001909.1 molecular chaperone YcdY -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - csgG / csgF / csgE / csgD / csgB / csgA / csgC 2836597..2872108 35511


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 14214.14 Da        Isoelectric Point: 6.4773

>T211356 WP_000854739.1 NZ_CP080246:2861786-2862166 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNDITLGRHPEEAKQ

Download         Length: 381 bp

>T211356 NZ_CP104848:186695-186802 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13791.44 Da        Isoelectric Point: 4.7511

>AT211356 WP_001285482.1 NZ_CP080246:2861365-2861739 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT211356 NZ_CP104848:c186638-186581 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2V687

Antitoxin

Source ID Structure
AlphaFold DB A0A0P7R0L9

References