Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2212320..2212938 Replicon chromosome
Accession NZ_CP080246
Organism Escherichia coli strain SYNB1934

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KZX95_RS10390 Protein ID WP_001291435.1
Coordinates 2212320..2212538 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KZX95_RS10395 Protein ID WP_000344800.1
Coordinates 2212564..2212938 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX95_RS10355 (2207608) 2207608..2208180 + 573 WP_000779831.1 YbaY family lipoprotein -
KZX95_RS10360 (2208211) 2208211..2208522 - 312 WP_000409908.1 MGMT family protein -
KZX95_RS10370 (2208901) 2208901..2209254 + 354 WP_000878140.1 DUF1428 domain-containing protein -
KZX95_RS10375 (2209296) 2209296..2210846 - 1551 WP_001528761.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KZX95_RS10380 (2211010) 2211010..2211480 - 471 WP_001304825.1 YlaC family protein -
KZX95_RS10385 (2211596) 2211596..2212147 - 552 WP_000102539.1 maltose O-acetyltransferase -
KZX95_RS10390 (2212320) 2212320..2212538 - 219 WP_001291435.1 HHA domain-containing protein Toxin
KZX95_RS10395 (2212564) 2212564..2212938 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KZX95_RS10400 (2213483) 2213483..2216632 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
KZX95_RS10405 (2216655) 2216655..2217848 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T211355 WP_001291435.1 NZ_CP080246:c2212538-2212320 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T211355 NZ_CP104848:186212-186319 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT211355 WP_000344800.1 NZ_CP080246:c2212938-2212564 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT211355 NZ_CP104848:c186163-186098 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References