Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 21265..21812 Replicon plasmid pW1316-1
Accession NZ_CP080236
Organism Escherichia coli O139:H1 strain W13-16

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag KZW92_RS24965 Protein ID WP_001384452.1
Coordinates 21534..21812 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag KZW92_RS24960 Protein ID WP_000079941.1
Coordinates 21265..21534 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW92_RS24940 (17632) 17632..17850 + 219 WP_001353651.1 heat-stable enterotoxin ST-I group a -
KZW92_RS24950 (19434) 19434..19826 - 393 Protein_23 IS21-like element IS21 family helper ATPase IstB -
KZW92_RS24955 (19826) 19826..20998 - 1173 WP_015056461.1 IS21-like element IS21 family transposase -
KZW92_RS24960 (21265) 21265..21534 + 270 WP_000079941.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
KZW92_RS24965 (21534) 21534..21812 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
KZW92_RS24970 (22121) 22121..23263 + 1143 WP_158649904.1 YiiX/YebB-like N1pC/P60 family cysteine hydrolase -
KZW92_RS25910 (23236) 23236..23340 + 105 Protein_28 hypothetical protein -
KZW92_RS24975 (23352) 23352..23657 + 306 WP_000482654.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - estIa 1..83443 83443
- inside IScluster/Tn - estIa 2395..29935 27540


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T211337 WP_001384452.1 NZ_CP080236:21534-21812 [Escherichia coli O139:H1]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T211337 NZ_CP104846:997465-997749 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT211337 WP_000079941.1 NZ_CP080236:21265-21534 [Escherichia coli O139:H1]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT211337 NZ_CP104846:997229-997462 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References