Detailed information of TA system TA211336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 1773..2308
• Replicon: plasmid pW1316-1
• Accession: NZ_CP080236
• Organism: Escherichia coli O139:H1 strain W13-16

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: KZW92_RS24845
• Protein ID: WP_258055396.1
• Coordinates: 2042..2308 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: G7QEH2
• Locus tag: KZW92_RS24840
• Protein ID: WP_015056448.1
• Coordinates: 1773..2042 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW92_RS24835 (1)	1..858	+	858	WP_219789730.1	incFII family plasmid replication initiator RepA	-
KZW92_RS24840 (1773)	1773..2042	+	270	WP_015056448.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
KZW92_RS24845 (2042)	2042..2308	+	267	WP_258055396.1	type II toxin-antitoxin system toxin YacB	Toxin
KZW92_RS24850 (2395)	2395..3567	+	1173	Protein_3	IS21-like element IS21 family transposase	-
KZW92_RS24855 (3567)	3567..4364	+	798	WP_042634526.1	IS21-like element IS21 family helper ATPase IstB	-
KZW92_RS24860 (4427)	4427..4708	+	282	Protein_5	IS66 family insertion sequence element accessory protein TnpB	-
KZW92_RS24865 (4728)	4728..6299	+	1572	WP_062873784.1	IS66-like element ISCro1 family transposase	-
KZW92_RS24870 (6334)	6334..6663	-	330	Protein_7	oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	estIa	1..83443	83443
	-	inside	IScluster/Tn	-	estIa	2395..29935	27540

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-85)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10301.82 Da
• Isoelectric Point: 8.0167

>T211336 WP_258055396.1 NZ_CP080236:2042-2308 [Escherichia coli O139:H1]
MEVFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVCQRQ

Nucleotide

• Length: 267 bp

>T211336 NZ_CP104846:838793-839080 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10226.54 Da
• Isoelectric Point: 7.2050

>AT211336 WP_015056448.1 NZ_CP080236:1773-2042 [Escherichia coli O139:H1]
MTQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVEQQHNTWFRDQVAAGRQQLERGDVLPHDMVESSATAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT211336 NZ_CP104846:839067-839369 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: G7QEH2