Detailed information of TA system TA211335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 4959743..4960448
• Replicon: chromosome
• Accession: NZ_CP080235
• Organism: Escherichia coli O139:H1 strain W13-16

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A3A6SJV3
• Locus tag: KZW92_RS24085
• Protein ID: WP_063085620.1
• Coordinates: 4960062..4960448 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KZW92_RS24080
• Protein ID: WP_001280945.1
• Coordinates: 4959743..4960072 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW92_RS24065 (4954900)	4954900..4955811	+	912	WP_001236019.1	glutathione ABC transporter permease GsiD	-
KZW92_RS24070 (4955989)	4955989..4958337	+	2349	WP_219789566.1	EAL domain-containing protein	-
KZW92_RS24075 (4958345)	4958345..4959673	+	1329	WP_000086873.1	GGDEF domain-containing protein	-
KZW92_RS24080 (4959743)	4959743..4960072	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
KZW92_RS24085 (4960062)	4960062..4960448	-	387	WP_063085620.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KZW92_RS24090 (4960674)	4960674..4961999	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
KZW92_RS24095 (4962212)	4962212..4962595	+	384	WP_000497137.1	biofilm formation regulator BssR	-
KZW92_RS24100 (4962706)	4962706..4963821	+	1116	WP_063085618.1	aldose sugar dehydrogenase YliI	-
KZW92_RS24105 (4963818)	4963818..4964444	-	627	WP_219789568.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14280.45 Da
• Isoelectric Point: 9.9296

>T211335 WP_063085620.1 NZ_CP080235:c4960448-4960062 [Escherichia coli O139:H1]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSTKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T211335 NZ_CP104846:642736-643050 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT211335 WP_001280945.1 NZ_CP080235:c4960072-4959743 [Escherichia coli O139:H1]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT211335 NZ_CP104846:643047-643463 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A3A6SJV3