Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4527029..4527647 Replicon chromosome
Accession NZ_CP080235
Organism Escherichia coli O139:H1 strain W13-16

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KZW92_RS22075 Protein ID WP_001291435.1
Coordinates 4527029..4527247 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KZW92_RS22080 Protein ID WP_000344800.1
Coordinates 4527273..4527647 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW92_RS22040 (4522318) 4522318..4522890 + 573 WP_000779826.1 YbaY family lipoprotein -
KZW92_RS22045 (4522921) 4522921..4523232 - 312 WP_000409911.1 MGMT family protein -
KZW92_RS22055 (4523611) 4523611..4523964 + 354 WP_000878140.1 DUF1428 domain-containing protein -
KZW92_RS22060 (4524006) 4524006..4525556 - 1551 WP_063085326.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KZW92_RS22065 (4525720) 4525720..4526190 - 471 WP_063085325.1 YlaC family protein -
KZW92_RS22070 (4526306) 4526306..4526857 - 552 WP_000102564.1 maltose O-acetyltransferase -
KZW92_RS22075 (4527029) 4527029..4527247 - 219 WP_001291435.1 HHA domain-containing protein Toxin
KZW92_RS22080 (4527273) 4527273..4527647 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KZW92_RS22085 (4528193) 4528193..4531342 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
KZW92_RS22090 (4531365) 4531365..4532558 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T211334 WP_001291435.1 NZ_CP080235:c4527247-4527029 [Escherichia coli O139:H1]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T211334 NZ_CP104846:c601420-600956 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT211334 WP_000344800.1 NZ_CP080235:c4527647-4527273 [Escherichia coli O139:H1]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT211334 NZ_CP104846:c601755-601420 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References