Detailed information of TA system TA211333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4321591..4323280
• Replicon: chromosome
• Accession: NZ_CP080235
• Organism: Escherichia coli O139:H1 strain W13-16

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A3A6SPN4
• Locus tag: KZW92_RS21130
• Protein ID: WP_063085362.1
• Coordinates: 4322360..4323280 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: KZW92_RS21125
• Protein ID: WP_063502601.1
• Coordinates: 4321591..4322373 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW92_RS25855 (4316896)	4316896..4317030	-	135	WP_258055208.1	hypothetical protein	-
KZW92_RS21120 (4318335)	4318335..4321307	-	2973	WP_258055210.1	type I restriction endonuclease	-
KZW92_RS21125 (4321591)	4321591..4322373	+	783	WP_063502601.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
KZW92_RS21130 (4322360)	4322360..4323280	+	921	WP_063085362.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
KZW92_RS21135 (4323714)	4323714..4324598	-	885	WP_001119037.1	LysR family transcriptional regulator	-
KZW92_RS21140 (4324863)	4324863..4325921	+	1059	WP_122987789.1	alpha/beta hydrolase	-
KZW92_RS21145 (4326063)	4326063..4327190	+	1128	WP_042028035.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34587.06 Da
• Isoelectric Point: 7.3238

>T211333 WP_063085362.1 NZ_CP080235:4322360-4323280 [Escherichia coli O139:H1]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDIERITTLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T211333 NZ_CP104846:289970-290497 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29094.32 Da
• Isoelectric Point: 8.1223

>AT211333 WP_063502601.1 NZ_CP080235:4321591-4322373 [Escherichia coli O139:H1]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGRPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVTSLTTELKIGDCQLAGSVPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT211333 NZ_CP104846:289697-289963 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A3A6SPN4