Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3940591..3941167 Replicon chromosome
Accession NZ_CP080235
Organism Escherichia coli O139:H1 strain W13-16

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag KZW92_RS19255 Protein ID WP_001297643.1
Coordinates 3940591..3940878 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag KZW92_RS19260 Protein ID WP_000063160.1
Coordinates 3940865..3941167 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW92_RS19235 (3936844) 3936844..3938124 - 1281 WP_001037400.1 DUF445 domain-containing protein -
KZW92_RS19240 (3938165) 3938165..3939397 - 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
KZW92_RS19245 (3939543) 3939543..3939638 - 96 Protein_3773 dienelactone hydrolase family protein -
KZW92_RS19250 (3939695) 3939695..3940392 + 698 Protein_3774 IS1 family transposase -
KZW92_RS19255 (3940591) 3940591..3940878 + 288 WP_001297643.1 BrnT family toxin Toxin
KZW92_RS19260 (3940865) 3940865..3941167 + 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
KZW92_RS19265 (3941201) 3941201..3942157 - 957 WP_001297640.1 GTPase -
KZW92_RS19270 (3942168) 3942168..3942371 - 204 WP_000467859.1 YbdD/YjiX family protein -
KZW92_RS19275 (3942421) 3942421..3944571 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T211331 WP_001297643.1 NZ_CP080235:3940591-3940878 [Escherichia coli O139:H1]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T211331 NZ_CP104846:186212-186319 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT211331 WP_000063160.1 NZ_CP080235:3940865-3941167 [Escherichia coli O139:H1]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT211331 NZ_CP104846:c186163-186098 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References