Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3703297..3704132 Replicon chromosome
Accession NZ_CP080235
Organism Escherichia coli O139:H1 strain W13-16

Toxin (Protein)


Gene name yeeV Uniprot ID A0A3A6S2N5
Locus tag KZW92_RS18035 Protein ID WP_000854800.1
Coordinates 3703297..3703674 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A3Z2YIX3
Locus tag KZW92_RS18040 Protein ID WP_032178229.1
Coordinates 3703764..3704132 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW92_RS18015 (3699389) 3699389..3700927 - 1539 WP_001187196.1 lysine decarboxylation/transport transcriptional activator CadC -
KZW92_RS18020 (3701676) 3701676..3702518 - 843 WP_063086391.1 DUF4942 domain-containing protein -
KZW92_RS18025 (3702603) 3702603..3702800 - 198 WP_001374283.1 DUF957 domain-containing protein -
KZW92_RS18030 (3702812) 3702812..3703300 - 489 WP_000779171.1 DUF5983 family protein -
KZW92_RS18035 (3703297) 3703297..3703674 - 378 WP_000854800.1 TA system toxin CbtA family protein Toxin
KZW92_RS18040 (3703764) 3703764..3704132 - 369 WP_032178229.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KZW92_RS18045 (3704182) 3704182..3704724 - 543 Protein_3534 antitoxin of toxin-antitoxin stability system -
KZW92_RS18050 (3704724) 3704724..3705257 - 534 Protein_3535 DUF945 domain-containing protein -
KZW92_RS18055 (3705585) 3705585..3708431 - 2847 WP_063086393.1 Ag43/Cah family autotransporter adhesin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3691900..3742034 50134


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14068.13 Da        Isoelectric Point: 9.1376

>T211330 WP_000854800.1 NZ_CP080235:c3703674-3703297 [Escherichia coli O139:H1]
MKTLPVLPGQAAGLRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDKPGF
NACTHSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHSEAKR

Download         Length: 378 bp

>T211330 NZ_CP104845:86468-86884 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13823.58 Da        Isoelectric Point: 6.3177

>AT211330 WP_032178229.1 NZ_CP080235:c3704132-3703764 [Escherichia coli O139:H1]
VSDTLHETNYPDDNNDRSWWGLPCTVTPCFRARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT211330 NZ_CP104845:86241-86471 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3A6S2N5

Antitoxin

Source ID Structure
AlphaFold DB A0A3Z2YIX3

References