Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2093200..2093854 Replicon chromosome
Accession NZ_CP080235
Organism Escherichia coli O139:H1 strain W13-16

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag KZW92_RS10450 Protein ID WP_000244781.1
Coordinates 2093447..2093854 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KZW92_RS10445 Protein ID WP_000354046.1
Coordinates 2093200..2093466 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW92_RS10425 (2089288) 2089288..2090721 - 1434 WP_063085817.1 6-phospho-beta-glucosidase BglA -
KZW92_RS10430 (2090766) 2090766..2091077 + 312 WP_001182952.1 N(4)-acetylcytidine aminohydrolase -
KZW92_RS10435 (2091241) 2091241..2091900 + 660 WP_000250269.1 hemolysin III family protein -
KZW92_RS10440 (2091977) 2091977..2092957 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
KZW92_RS10445 (2093200) 2093200..2093466 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KZW92_RS10450 (2093447) 2093447..2093854 + 408 WP_000244781.1 protein YgfX Toxin
KZW92_RS10455 (2093894) 2093894..2094415 - 522 WP_001055888.1 flavodoxin FldB -
KZW92_RS10460 (2094527) 2094527..2095423 + 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
KZW92_RS10465 (2095448) 2095448..2096158 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KZW92_RS10470 (2096164) 2096164..2097897 + 1734 WP_000813177.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T211325 WP_000244781.1 NZ_CP080235:2093447-2093854 [Escherichia coli O139:H1]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T211325 NZ_CP104843:c4517236-4517063 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGCGCATTCCTGGTCGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT211325 WP_000354046.1 NZ_CP080235:2093200-2093466 [Escherichia coli O139:H1]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT211325 NZ_CP104843:c4517560-4517264 [Escherichia coli]
ATGGAAGGTAAAAATAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References