Detailed information of TA system TA021132

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 59992..61577
• Replicon: chromosome
• Accession: NC_006512
• Organism: Idiomarina loihiensis L2TR

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q5QZX8
• Locus tag: IL_RS00305
• Protein ID: WP_011233322.1
• Coordinates: 60264..61577 (+)
• Length: 438 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: IL_RS00300
• Protein ID: WP_011233321.1
• Coordinates: 59992..60264 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IL_RS00275	55214..56110	+	897	WP_011233317.1	LysR family transcriptional regulator	-
IL_RS00280	56928..57470	+	543	WP_011233318.1	sigma-70 family RNA polymerase sigma factor	-
IL_RS00285	57460..58314	+	855	WP_011233319.1	hypothetical protein	-
IL_RS00290	58709..59119	-	411	WP_011233320.1	helix-turn-helix transcriptional regulator	-
IL_RS00295	59279..59602	+	324	WP_081423212.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
IL_RS00300	59992..60264	+	273	WP_011233321.1	helix-turn-helix domain-containing protein	Antitoxin
IL_RS00305	60264..61577	+	1314	WP_011233322.1	type II toxin-antitoxin system HipA family toxin	Toxin
IL_RS00310	61796..62305	-	510	WP_011233323.1	helix-turn-helix transcriptional regulator	-
IL_RS00315	62404..63600	+	1197	WP_011233324.1	TolC family protein	-
IL_RS00320	63597..64637	+	1041	WP_011233325.1	membrane fusion-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (150-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-55)

Sequences

Toxin

Protein

• Length: 438 a.a.
• Molecular weight: 49062.80 Da
• Isoelectric Point: 6.1465

>T21132 WP_011233322.1 NC_006512:60264-61577 [Idiomarina loihiensis L2TR]
MNTLDVYMNGYRVGHLSRNDSGAHTFRYDEMWIDKEGARPISLSMPLRHDAYSGREVINFFDNLLPDNEEVRDRIVARYQ
ADSDQAFDLLAAIGKDSVGALQFVPAGEKPGNIRELRYEKLTEEKLERVLKGYQSKAPLGMLRELDDFRISIAGAQEKTA
LLKIDGAWCIPNGNTPTTHIIKLPIGKIQSHDHTIDLSDSVENEMLCLKIARAFGFEAPDCEVIITPNVKALAIERFDRT
YSSDHSWLMRLPQEDYCQVLGLPSARKYESDNGPSIASIMKHLQGSADSTGDREAFMGAQVLFFLLGATDGHGKNFSVHI
EPYGAFRLTPLYDIISAYTAVGGTGINIRDLRLAMSLRGAGGKKRNIYQIFKRHFLNAAKEAEFSPTRMSEIINALCDQV
DDVIERVKGDLPNDFPTRVSTPIFDGMQERKRRLLIE

Nucleotide

• Length: 1314 bp

>T21132 NC_006512:60264-61577 [Idiomarina loihiensis L2TR]
ATGAATACGCTCGACGTCTATATGAACGGCTACCGCGTAGGTCACCTGAGTCGAAATGATTCCGGCGCTCACACATTTCG
TTATGATGAGATGTGGATTGATAAAGAGGGTGCACGGCCAATCTCTTTGTCTATGCCCTTACGCCATGACGCTTATTCAG
GGCGCGAGGTTATTAACTTTTTTGATAACCTGCTGCCTGACAATGAGGAAGTTAGAGACCGGATTGTTGCCCGTTATCAA
GCCGACTCCGATCAAGCATTTGATCTACTGGCGGCTATTGGGAAAGATAGTGTTGGTGCATTGCAGTTCGTCCCGGCAGG
AGAAAAACCAGGGAATATTCGTGAACTGCGTTATGAAAAACTCACCGAAGAGAAGTTAGAACGTGTTTTAAAAGGCTACC
AGTCCAAGGCACCTTTGGGCATGCTTCGGGAGTTGGATGATTTTAGAATATCAATTGCTGGGGCTCAGGAAAAAACAGCC
CTGTTAAAAATAGATGGTGCATGGTGTATTCCAAATGGAAACACGCCGACTACTCATATCATTAAACTTCCGATTGGTAA
GATTCAAAGCCACGATCACACAATCGATTTAAGTGACAGCGTTGAAAATGAAATGCTCTGCTTAAAAATTGCACGAGCAT
TCGGTTTTGAAGCACCGGATTGTGAGGTAATTATCACACCAAATGTTAAAGCCCTCGCCATTGAGCGTTTTGATCGTACA
TATTCAAGTGATCATTCTTGGTTAATGCGGCTTCCTCAAGAAGATTATTGTCAGGTGCTTGGACTGCCATCTGCGCGAAA
GTACGAATCGGATAACGGTCCGTCGATTGCCAGTATTATGAAGCATCTTCAAGGGTCTGCTGATTCCACAGGTGATCGTG
AGGCTTTCATGGGCGCACAGGTGCTTTTCTTTCTATTAGGCGCCACTGATGGGCACGGAAAGAATTTCTCTGTGCATATT
GAGCCTTATGGGGCTTTCCGTTTAACGCCTTTGTACGACATTATTTCTGCTTATACTGCTGTGGGTGGAACAGGCATCAA
TATTCGCGACTTGAGACTTGCGATGAGCTTGCGAGGGGCGGGGGGTAAAAAGCGGAACATCTATCAAATTTTTAAACGTC
ATTTTTTAAACGCAGCGAAAGAAGCTGAATTCAGTCCGACCAGAATGAGTGAAATTATCAACGCCCTGTGTGATCAGGTT
GACGATGTTATTGAACGAGTAAAAGGCGATTTACCCAATGATTTCCCGACAAGGGTTTCGACACCAATCTTTGATGGTAT
GCAAGAGCGGAAACGCCGACTACTTATCGAATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10291.87 Da
• Isoelectric Point: 8.5607

>AT21132 WP_011233321.1 NC_006512:59992-60264 [Idiomarina loihiensis L2TR]
MKITDPKALSIYLRDERRRRGLSQAKVGDLVGLRQGTVSKFESMPEKMQLDTLFRLLSALELELEVKPKGTVLKKNVDSS
TPGDEWTEEW

Nucleotide

• Length: 273 bp

>AT21132 NC_006512:59992-60264 [Idiomarina loihiensis L2TR]
ATGAAAATTACTGATCCCAAAGCATTATCGATCTACCTACGGGATGAACGAAGACGTCGCGGCCTGAGTCAAGCTAAGGT
TGGTGATTTAGTCGGACTTAGGCAGGGGACGGTCTCTAAGTTTGAATCAATGCCAGAGAAAATGCAGCTCGATACGTTGT
TTCGCTTGCTGTCCGCTTTAGAGCTCGAGCTGGAAGTGAAACCTAAAGGCACCGTTCTGAAGAAGAACGTTGATTCGTCA
ACGCCTGGTGATGAATGGACTGAGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: Q5QZX8