Detailed information of TA system TA211314

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 749159..750747
• Replicon: chromosome
• Accession: NZ_CP080235
• Organism: Escherichia coli O139:H1 strain W13-16

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A3A6RNL0
• Locus tag: KZW92_RS03815
• Protein ID: WP_063085289.1
• Coordinates: 749159..750481 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KZW92_RS03820
• Protein ID: WP_001296726.1
• Coordinates: 750481..750747 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW92_RS03775 (744208)	744208..744779	-	572	Protein_749	NAD(P)H-dependent oxidoreductase	-
KZW92_RS03780 (744929)	744929..745081	-	153	WP_001314732.1	amidohydrolase family protein	-
KZW92_RS03785 (745071)	745071..745490	-	420	WP_001314733.1	amidohydrolase family protein	-
KZW92_RS03790 (745450)	745450..745605	-	156	WP_001327998.1	hypothetical protein	-
KZW92_RS03795 (745708)	745708..746655	-	948	WP_063085290.1	aldo/keto reductase	-
KZW92_RS03800 (746717)	746717..747406	-	690	WP_000801314.1	type 1 glutamine amidotransferase domain-containing protein	-
KZW92_RS03805 (747415)	747415..747723	-	309	WP_001221046.1	hypothetical protein	-
KZW92_RS03810 (747863)	747863..748761	+	899	Protein_756	LysR family transcriptional regulator	-
KZW92_RS03815 (749159)	749159..750481	-	1323	WP_063085289.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
KZW92_RS03820 (750481)	750481..750747	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49313.91 Da
• Isoelectric Point: 8.5849

>T211314 WP_063085289.1 NZ_CP080235:c750481-749159 [Escherichia coli O139:H1]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDHVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T211314 NZ_CP104843:2878196-2878303 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT211314 WP_001296726.1 NZ_CP080235:c750747-750481 [Escherichia coli O139:H1]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT211314 NZ_CP104843:c2878143-2878082 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A3A6RNL0