Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 651196..651834 Replicon chromosome
Accession NZ_CP080235
Organism Escherichia coli O139:H1 strain W13-16

Toxin (Protein)


Gene name hicA Uniprot ID A0A3A6S5Q3
Locus tag KZW92_RS03375 Protein ID WP_063085535.1
Coordinates 651196..651372 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag KZW92_RS03380 Protein ID WP_001270286.1
Coordinates 651418..651834 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW92_RS03355 (646819) 646819..647990 - 1172 Protein_665 BenE family transporter YdcO -
KZW92_RS03360 (648082) 648082..648618 + 537 WP_000429507.1 DNA-binding transcriptional regulator SutR -
KZW92_RS03365 (648691) 648691..650652 + 1962 WP_063085534.1 23S rRNA 5-hydroxycytidine C2501 synthase -
KZW92_RS03370 (650744) 650744..650974 - 231 WP_000494244.1 YncJ family protein -
KZW92_RS03375 (651196) 651196..651372 + 177 WP_063085535.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
KZW92_RS03380 (651418) 651418..651834 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
KZW92_RS03385 (651913) 651913..653319 + 1407 WP_063085536.1 PLP-dependent aminotransferase family protein -
KZW92_RS03390 (653564) 653564..654709 + 1146 WP_000047450.1 ABC transporter substrate-binding protein -
KZW92_RS03395 (654727) 654727..655740 + 1014 WP_063085537.1 ABC transporter ATP-binding protein -
KZW92_RS03400 (655741) 655741..656682 + 942 WP_063085538.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6751.78 Da        Isoelectric Point: 11.5666

>T211313 WP_063085535.1 NZ_CP080235:651196-651372 [Escherichia coli O139:H1]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPNDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T211313 NZ_CP104843:c2638067-2637891 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTGAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT211313 WP_001270286.1 NZ_CP080235:651418-651834 [Escherichia coli O139:H1]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT211313 NZ_CP104843:c2637845-2637429 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAACAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3A6S5Q3

Antitoxin

Source ID Structure

References