Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4107760..4108414 Replicon chromosome
Accession NZ_CP080233
Organism Escherichia coli O139:H1 strain P15-25

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag KZW90_RS20060 Protein ID WP_000244781.1
Coordinates 4107760..4108167 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KZW90_RS20065 Protein ID WP_000354046.1
Coordinates 4108148..4108414 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW90_RS20040 (4103717) 4103717..4105450 - 1734 WP_000813177.1 single-stranded-DNA-specific exonuclease RecJ -
KZW90_RS20045 (4105456) 4105456..4106166 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KZW90_RS20050 (4106191) 4106191..4107087 - 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
KZW90_RS20055 (4107199) 4107199..4107720 + 522 WP_001055888.1 flavodoxin FldB -
KZW90_RS20060 (4107760) 4107760..4108167 - 408 WP_000244781.1 protein YgfX Toxin
KZW90_RS20065 (4108148) 4108148..4108414 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KZW90_RS20070 (4108657) 4108657..4109637 + 981 WP_000886050.1 tRNA-modifying protein YgfZ -
KZW90_RS20075 (4109714) 4109714..4110373 - 660 WP_000250269.1 hemolysin III family protein -
KZW90_RS20080 (4110537) 4110537..4110848 - 312 WP_001182952.1 N(4)-acetylcytidine aminohydrolase -
KZW90_RS20085 (4110893) 4110893..4112326 + 1434 WP_063085817.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T211306 WP_000244781.1 NZ_CP080233:c4108167-4107760 [Escherichia coli O139:H1]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T211306 NZ_CP104843:253244-253351 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT211306 WP_000354046.1 NZ_CP080233:c4108414-4108148 [Escherichia coli O139:H1]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT211306 NZ_CP104843:c253195-253132 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References