Detailed information of TA system TA211294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2562622..2564210
• Replicon: chromosome
• Accession: NZ_CP080233
• Organism: Escherichia coli O139:H1 strain P15-25

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A3A6RNL0
• Locus tag: KZW90_RS12470
• Protein ID: WP_063085289.1
• Coordinates: 2562622..2563944 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KZW90_RS12475
• Protein ID: WP_001296726.1
• Coordinates: 2563944..2564210 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW90_RS12430 (2557666)	2557666..2558237	-	572	Protein_2433	NAD(P)H-dependent oxidoreductase	-
KZW90_RS12435 (2558387)	2558387..2558539	-	153	WP_001314732.1	amidohydrolase family protein	-
KZW90_RS12440 (2558529)	2558529..2558948	-	420	WP_001314733.1	amidohydrolase family protein	-
KZW90_RS12445 (2558908)	2558908..2559063	-	156	WP_001327998.1	hypothetical protein	-
KZW90_RS12450 (2559166)	2559166..2560113	-	948	WP_063085290.1	aldo/keto reductase	-
KZW90_RS12455 (2560175)	2560175..2560864	-	690	WP_000801314.1	type 1 glutamine amidotransferase domain-containing protein	-
KZW90_RS12460 (2560873)	2560873..2561181	-	309	WP_001221046.1	hypothetical protein	-
KZW90_RS12465 (2561321)	2561321..2562219	+	899	Protein_2440	LysR family transcriptional regulator	-
KZW90_RS12470 (2562622)	2562622..2563944	-	1323	WP_063085289.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
KZW90_RS12475 (2563944)	2563944..2564210	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49313.91 Da
• Isoelectric Point: 8.5849

>T211294 WP_063085289.1 NZ_CP080233:c2563944-2562622 [Escherichia coli O139:H1]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDHVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T211294 NZ_CP104836:c2713454-2713269 [Enterobacter cloacae]
GTGAAAAGTGCGGATGTCATTACTGTTCTGGTCAGGCATGGCTGGAAATGTGTAAGAACGAAAGGAAGCCATCATCAGTT
TCGCCACCCAGTACATGCGGGGTTGGTGACCGTTCCACATCCCAAAAAGGACATAAAACCCGGCACCCTCGCGCAAATCT
GGCGGCAGGCGGGAATCAAACACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT211294 WP_001296726.1 NZ_CP080233:c2564210-2563944 [Escherichia coli O139:H1]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT211294 NZ_CP104836:c2713254-2712850 [Enterobacter cloacae]
ATGTTTTATCCGGCTTACATTCATTCTGATCCCGACGGTAGCGCCAGCGGTTTTTTTCCTGACGTTCCTGGTTGTTTTTT
TGCCGGCAATTCTCTGGATGATGCTTTTCAGGATGCACGTGATGCACTGACAGCTCACTTCGAAGCGCTATTTGAAATGG
ATGAAGAGTTACCTCTCCCTGGCAATGTTGAAGCACATCTTGAAGCCACACCGGGTGATTTTATCGGCGGACAATGGTTG
CTTGTAGATATCAATATGAAGCAATTCGACGGTAAGGCCGAGCGTATCAATATCACCCTGCCTCGTCGATTGCTGGTGAA
AATTGATTCTTTTGTGAATGAGCATCCGCAGTTTAGTAACCGAAGCGCTTTTCTTGCTGAGGCAGCGCGTCGCGTGCTGC
CTTAA

Structures

Toxin

• AlphaFold DB: A0A3A6RNL0