Detailed information of TA system TA211293

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2470276..2470914
• Replicon: chromosome
• Accession: NZ_CP080233
• Organism: Escherichia coli O139:H1 strain P15-25

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3A6S5Q3
• Locus tag: KZW90_RS12045
• Protein ID: WP_063085535.1
• Coordinates: 2470276..2470452 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KZW90_RS12050
• Protein ID: WP_001270286.1
• Coordinates: 2470498..2470914 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW90_RS12025 (2465899)	2465899..2467070	-	1172	Protein_2351	BenE family transporter YdcO	-
KZW90_RS12030 (2467162)	2467162..2467698	+	537	WP_000429507.1	DNA-binding transcriptional regulator SutR	-
KZW90_RS12035 (2467771)	2467771..2469732	+	1962	WP_063085534.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KZW90_RS12040 (2469824)	2469824..2470054	-	231	WP_000494244.1	YncJ family protein	-
KZW90_RS12045 (2470276)	2470276..2470452	+	177	WP_063085535.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KZW90_RS12050 (2470498)	2470498..2470914	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KZW90_RS12055 (2470993)	2470993..2472399	+	1407	WP_063085536.1	PLP-dependent aminotransferase family protein	-
KZW90_RS12060 (2472644)	2472644..2473789	+	1146	WP_000047450.1	ABC transporter substrate-binding protein	-
KZW90_RS12065 (2473807)	2473807..2474820	+	1014	WP_063085537.1	ABC transporter ATP-binding protein	-
KZW90_RS12070 (2474821)	2474821..2475762	+	942	WP_063085538.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T211293 WP_063085535.1 NZ_CP080233:2470276-2470452 [Escherichia coli O139:H1]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPNDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T211293 NZ_CP104836:c2327408-2327124 [Enterobacter cloacae]
ATGACTTATGAACTGGAATTTGACCCGAGGGCGTTAAAAGAGTGGAGCAAGCTGGGGGAGACTGTAAAAAAGCAGTTCAG
GAAGAAACTTGCCGGGGTGTTGGTAAACCCCCGGATCGAATCGGCCCGTCTGCATAATCTGCCTGATTGCTACAAAATAA
AACTCAGATCCCAGGGTTACCGTTTGGTCTATCAGGTTCAGGACAACGTGGTAACGGTAGTTGTCGTCGCCATTGGGAAA
AGAGAGAAATCAGCCGTATATCACGATGCTAACAAACGGCTATAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT211293 WP_001270286.1 NZ_CP080233:2470498-2470914 [Escherichia coli O139:H1]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT211293 NZ_CP104836:c2327640-2327398 [Enterobacter cloacae]
ATGGCTACGCTTAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTGGAAAAACTCAATATCACCCC
AACAGAAGCCGTGCGACTCCTTTTTCAGTACGTTGCGGAGACGGGGCGCATGCCGGTGAAAACGGTCACGATTAGCGACA
GCGAAGATGCGCTGATACAGACAGTCCGGGAGCGACTGGCTAATCCGCAAAAGGGAATTAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A3A6S5Q3