Detailed information of TA system TA211287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1734311..1735016
• Replicon: chromosome
• Accession: NZ_CP080233
• Organism: Escherichia coli O139:H1 strain P15-25

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A3A6SJV3
• Locus tag: KZW90_RS08255
• Protein ID: WP_063085620.1
• Coordinates: 1734630..1735016 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KZW90_RS08250
• Protein ID: WP_219804798.1
• Coordinates: 1734311..1734640 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW90_RS08235 (1729468)	1729468..1730379	+	912	WP_001236019.1	glutathione ABC transporter permease GsiD	-
KZW90_RS08240 (1730557)	1730557..1732905	+	2349	WP_021523051.1	EAL domain-containing protein	-
KZW90_RS08245 (1732913)	1732913..1734241	+	1329	WP_000086873.1	GGDEF domain-containing protein	-
KZW90_RS08250 (1734311)	1734311..1734640	-	330	WP_219804798.1	DNA-binding transcriptional regulator	Antitoxin
KZW90_RS08255 (1734630)	1734630..1735016	-	387	WP_063085620.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KZW90_RS08260 (1735242)	1735242..1736567	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
KZW90_RS08265 (1736780)	1736780..1737163	+	384	WP_000497137.1	biofilm formation regulator BssR	-
KZW90_RS08270 (1737274)	1737274..1738389	+	1116	WP_063085618.1	aldose sugar dehydrogenase YliI	-
KZW90_RS08275 (1738386)	1738386..1739012	-	627	WP_001314584.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14280.45 Da
• Isoelectric Point: 9.9296

>T211287 WP_063085620.1 NZ_CP080233:c1735016-1734630 [Escherichia coli O139:H1]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSTKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T211287 NZ_CP104835:2701046-2701231 [Achromobacter xylosoxidans]
ATGAAATACAGCGAATTCAAAAAATGGCTTGAGCGGCAAGGAGCGGTTTTTGTTGCGCATCGTTCCGGAAGCAGCCATTT
TCGGGTCACGTTGAACGGCGCCACGACGATCTTTCCGTACCATGGCTCCAAGGAAATGGGTAAACACCTTGAACATGAAA
TCAAGAAGCAGCTCGGATTGAAATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11964.04 Da
• Isoelectric Point: 10.0101

>AT211287 WP_219804798.1 NZ_CP080233:c1734640-1734311 [Escherichia coli O139:H1]
MSDKLQMMLEDAKELNALGLISDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT211287 NZ_CP104835:2701307-2701726 [Achromobacter xylosoxidans]
ATGCTGACCTATCCGTTCACCCTGACGCTTGATTCCAATCGCACCTGGCTCGTTCGATTTCCAGATATTCCGGAAGCGTT
GACGGTTGGTGACGACGAGCAAGAGGCCGCGATCAACGCCAGGGAAGCGCTTGAGGCTGCGCTGGAAATCTACTTTGACG
CGCGCCGCCCCATTCCGCTGCCGTCGCCCGTATCGGCGGGGCAGGCGTCGGTGACCTTGCCGGCACTGATCACCAGCAAG
GTGTTCCTGTCCAACGAGATGATCCAGCAAGGTGTGCGCAAGGCGGAGTTGGCTCGCCGCATGGGCGTGCACATGCCGCA
AGTGGATCGTCTGCTGGATGTGCGGCACGCATCCCGGATCGAGCAGGTCGAGTCGGCGTTGGAGCAGCTTGGGCGGCGCC
TGGAGGTGTCGCTGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A3A6SJV3